农业研究与应用2026，Vol.39Issue(2)：175-189,15.DOI:10.20191/j.cnki.2095-0764.2026.02.002
盐胁迫下芒果幼株叶片转录组分析与耐盐基因挖掘
Transcriptome Analysis and Salt-Tolerance Gene Mining in Mango Seedling Leaves under Salt Stress
摘要
Abstract
[Objective]Soil salinization is a major constraint in mango(Mangifera indica L.)production,particularly in high-temperature,low-rainfall regions like Baise,Guangxi,where drought often exacerbates soil salinization,severely impacting mango yield.This study aimed to elu-cidate the molecular response mechanisms of mango seedling leaves under salt stress through tran-scriptome sequencing,identify candidate salt-tolerance genes,and provide a molecular basis for mango stress-resistant breeding to enhance adaptation to saline-alkali soils and promote sustainable development of the mango industry.[Method]Six Months old Tainung No.1 mango seedlings were treated with 0.5 mol/L NaCl,and top leaf samples were collected at 0 h(CK),24 h(SaltD1),48 h(SaltD2)and 72 h(SaltD3),respectively.The samples were flash-frozen in liquid nitrogen,total RNA was extracted,and cDNA libraries were constructed.Double-end sequencing was performed using the Illumina HiSeq platform.After quality control,the clean reads were aligned to the refer-ence genome(GCF_011075055.1)using HISAT2,and the transcripts were assembled with String-Tie.Differentially expressed genes(DEGs)were identified using DESeq2(|log2FC|＞1,FDR＜0.05),and GO,KEGG enrichment analyses were conducted with eggnog-mapper(FDR＜0.05)to reveal the transcription factor expression patterns.[Result]The sequencing data showed high quali-ty,with an average of 43915377 effective sequences,Q20＞96%,and genome alignment rates of 93.46%～95.13%.The DEGs increased with stress duration extension,among them 2 430 were up-regulated and 2 896 down-regulated.GO enrichment showed up-regulated DEGs associated with salt response,water stress,sulfate assimilation pathways,while down-regulated DEGs were linked to photosynthesis-related pathways,such as chlorophyll binding and thylakoid structure.KEGG analysis revealed the up-regulated pathways including amino sugar metabolism and MAPK signal-ing,and the down-regulated pathways involving photosynthetic antenna proteins and porphyrin me-tabolism.Transcription factor analysis indicated NAC,EREBP,and ABFfamilies were upregulat-ed,regulating antioxidant and osmotic responses,while MYBPand HD-ZIPfamilies were down-regulated,affecting growth and development.[Conclusion]This study revealed the mechanisms of ion homeostasis,photosynthetic suppression,and metabolic regulation in mango leaves under salt stress,and identified NACand other transcription factors as potential salt-tolerance genes.Future research should integrate multi-omics and field trials to optimize the salt-tolerance breeding strate-gies,and enhance the stress resistance and yield in mango.关键词
芒果/盐胁迫/转录组测序/差异表达基因/转录因子Key words
Mango/salt stress/transcriptome sequencing/differentially expressed gene/tran-scription factor分类
农业科技引用本文复制引用
吕玲玲,曾梅连,谭姗姗,李曦,朱正杰,施明德..盐胁迫下芒果幼株叶片转录组分析与耐盐基因挖掘[J].农业研究与应用,2026,39(2):175-189,15.基金项目
广西自然科学基金项目(2022GXNSFBA035458 （）
2023GXNSFAA026451 （）
2025GXNSFAA069977) （）
大学生创新训练区级项目(202310609155). （202310609155）
