化学试剂2026，Vol.48Issue(3)：42-50,9.DOI:10.13822/j.cnki.hxsj.2026.0022
肝缺血再灌注损伤可视化荧光探针的研究进展
Advances in Fluorescent Probes for Visualizing Hepatic Ischemia-Reperfusion Injury
摘要
Abstract
Hepatic ischemia-reperfusion injury(HIRI)is a severe complication in surgeries,such as hepatectomy and liver transplantation,and involves a complex cascade of ischemia-induced energy failure,oxidative stress,calcium overload,and cell death upon reperfusion.Conventional diagnostic approaches,including serologic assays and imaging techniques,are limited by low sensitivity,poor spatiotemporal resolution,and invasiveness,thereby restricting early and in situ detection of critical biomarkers.Fluorescent probe technology has emerged as a powerful tool for studying HIRI owing to its high sensitivity,spatiotemporal resolution,and real-time imaging capabilities.This review first outlined the pathological progression of HIRI and the key biomarkers implicated in this process,including reactive oxygen species(ROS),microenvironmental parameters(e.g.,viscosity),energy metabolites(e.g.,ATP and mitochondrial DNA),and gas signaling molecules(e.g.,H2S).Subsequently,we systematically summarized recent advances in the design and application of fluorescent probes that target these biomarkers.These probes were categorized and discussed based on their detection targets and design strategies,including probes for ROS,energy metabolism,microenvironmental parameters,gas signaling molecules,and near-infrared and multi-responsive probes,with a focus on their design principles,response mechanisms,imaging performance,and representative applications.Finally,we addressed the current challenges in reversible detection,multi-analyte imaging,and clinical translation while highlighting future applications of fluorescent probes in the early diagnosis,mechanistic research,drug evaluation,and surgical guidance of HIRI.关键词
缺血再灌注损伤/荧光探针/生物标志物/靶向性/近红外Key words
ischemia-reperfusion injury/fluorescent probes/biomarkers/targeting/near-infrared imaging分类
化学化工引用本文复制引用
张文轩,徐黎,冯书敏,李玉桃..肝缺血再灌注损伤可视化荧光探针的研究进展[J].化学试剂,2026,48(3):42-50,9.基金项目
湖北省联合基金项目(2023AFD125). （2023AFD125）
