华北农学报2026，Vol.41Issue(1)：1-13,13.DOI:10.7668/hbnxb.20196073
基于QTL定位与组学分析挖掘水稻粒质量及粒形候选基因
Identification of Candidate Genes for Rice Grain Weight and Shape via QTL Mapping and Omics Analysis
摘要
Abstract
Based on quantitative trait locus(QTL)mapping of grain weight and grain shape-related traits in a backcross inbred line(BIL)population derived from the cross between indica rice variety Changhui 891 and japoni-ca rice variety 02428,this study provided valuable genetic resources for elucidating the genetic basis of grain devel-opment and molecular breeding for high yield and quality in rice.A high-density genetic map was used to conduct QTL analysis for thousand-grain weight,grain length,grain width,grain thickness,and length-width ratio under two environments.Transcriptome data of developing grains at 5,10,and 15 days after flowering from both parents were integrated to analyze candidate genes within QTL regions.Frequency distribution indicated that grain weight and shape traits in the BIL population exhibited typical quantitative characteristics,confirming the suitability for QTL mapping.A total of 37 QTLs were detected across both environments,including 18 specific to Hainan(Sanya),12 specific to Jiangxi(Nanchang),and 7 consistently identified in both locations.These QTLs were distributed on chro-mosomes 1-5,7,8,11,and 12,comprising 7 for thousand grains weight,5 for grain length,7 for grain width,11 for length-width ratio,and 7 for grain thickness.The analysis showed that the LOD values of these QTLs ranged from 2.79 to 43.10,with a contribution rate of 1.36％to 46.12％.By integrating differentially expressed genes from de-veloping grains of both parents at three stages,28 candidate genes were prioritized from the 37 QTL regions.Among these,LOC_Os11g10480 and LOC_Os11g10100 were identified as key candidates for further functional validation.关键词
水稻/回交重组自交系/粒形/千粒质量/QTLKey words
Rice/BIL/Grain shape/Thousand-grain weight/QTL分类
农业科技引用本文复制引用
钟奇,Mvuyeni Nyasulu,曹国梁,边建民..基于QTL定位与组学分析挖掘水稻粒质量及粒形候选基因[J].华北农学报,2026,41(1):1-13,13.基金项目
国家自然科学基金项目(32160485) （32160485）
江西省引进培养创新创业高层次人才"千人计划"项目(jxsq2023201057) （jxsq2023201057）
江西省农业技术项目(JXNK202301-03) （JXNK202301-03）
