华北农学报2026，Vol.41Issue(1)：14-21,8.DOI:10.7668/hbnxb.20196288
寒地粳稻种质资源香味基因Badh2变异分析及其KASP标记开发与应用
Analysis of the Fragrance Gene Badh2 Variation in Cold-resistant Japonica Rice Germplasm Resources and the Development and Application of KASP Markers
摘要
Abstract
The screening of aromatic rice germplasm resources and the rapid identification and utilization of ar-omatic genes have practical significance for the genetic breeding of aromatic rice.In order to clarify the aroma types of aromatic rice materials in Heilongjiang Province and improve the breeding efficiency of aromatic rice varieties,180 aromatic rice varieties approved and promoted in Heilongjiang Province were sequenced.Compared with the ref-erence genome Nipponbare,the sequencing results were divided into two types of mutations.The first type was the 8 bp deletion of exon 7 and three base substitutions(Badh2-E7 type),including 177 varieties.The second type was the 7 bp deletion of exon 2(Badh2-E2 type),which contained only three varieties.According to these two mutation types,two specific KASP molecular markers were designed to accurately detect the aroma types of E7 and E2 in rice.Through the identification of 180 aromatic rice varieties,the typing results were consistent with the sequencing results.Two hybrid populations were constructed by using E7 aromatic rice variety Wuyoudao 4 and E2 aromatic rice variety Songkejing 134 with non-aromatic japonica rice varieties Weinong 101 and Dongfu 114,respectively.The F2 generation of the hybrid population was screened and identified for aroma genes,and the identification results were consistent with 100％,which verified the accuracy of KASP molecular markers.The 620 japonica rice parents created and collected by our laboratory were further identified by KASP markers,and 248 aromatic rice materials were screened.It was found that these parents were all Badh2-E7 type genes.At the same time,the existing 1 220 F7 generation materials with E7 aromatic rice as parents were genotyped,including 293 aromatic rice materials,906 non-aromatic rice materials and 21 heterozygous rice materials.Five aromatic and non-aromatic rice materials were randomly selected for sequencing verification.The sequencing results verified that the KASP molecular marker typ-ing was correct.This study provided new markers and materials for breeding new fragrant rice varieties in cold re-gions by using molecular marker assisted selection.关键词
水稻/香味基因/Badh2/KASP/分子标记辅助选择Key words
Rice/Fragrance gene/Badh2/KASP/Molecular marker-assisted selection分类
农业科技引用本文复制引用
刘长林,辛威,吴梦,王敬国,刘化龙,杨洛淼,徐善斌,邹德堂,郑洪亮..寒地粳稻种质资源香味基因Badh2变异分析及其KASP标记开发与应用[J].华北农学报,2026,41(1):14-21,8.基金项目
黑龙江省自然科学基金优秀青年项目(YQ2024C007) （YQ2024C007）
国家自然科学基金面上项目(32472201) （32472201）
