华北农学报2026，Vol.41Issue(1)：49-55,7.DOI:10.7668/hbnxb.20195943
控制玉米叶片数的QTL定位及基因功能注释
QTL Mapping and Gene Functional Annotation for Controlling Maize Leaf Number
摘要
Abstract
To clarify the genetic characteristics of total leaf number and leaf number above the ear in maize,and reveal their regulatory effects on maize yield potential,biomass accumulation,and lodging resistance,experi-ments on QTL mapping and gene mining for controlling maize leaf number were conducted.By using SLAF-seq tech-nology for high-throughput sequencing of Chang 7-2,PHB1M,and 138 F2∶3 families,combined with two planting density treatments(E1:60 000 plants/ha;E2:120 000 plants/ha)QTL mapping was performed on leaf number phe-notypic data,and gene mining was performed on the mapping results.The results showed that two total leaf number QTLs distributed on chromosome 8 were the main effect QTLs,contribution rates were 16.96％and 23.08％,re-spectively,and the additive effect value was negative;the QTL for the leaf number above the ear distributed on chro-mosomes 2 and 4,with a positive additive effect value.the two QTLs distributed on chromosome 4 were the main effect QTL,contribution rates were 10.18％and 14.75％,respectively;the QTLs for total leaf number and leaf number above the ear were distributed in different chromosomal regions and might be subject to relatively independ-ent genetic regulation.Gene functional analysis revealed genes screened involvement in carbohydrate metabolism,plant hormone signal transduction,and selected some transcription factors.The results of this study provide richer theoretical support for further revealing the genetic basis of maize leaf number and offer targets for marker-assisted breeding.关键词
玉米/总叶片数/穗上叶片数/QTL/基因挖掘Key words
Maize/Total leaf number/Leaf number above the ear/QTL/Gene mining分类
农业科技引用本文复制引用
薛春雷,吴慧,韩平安,马岩,王永行,孙峰成,张旭婷,张海龙,付增娟,刘亚楠,吴海燕,张子玉,张赛楠,余忠浩..控制玉米叶片数的QTL定位及基因功能注释[J].华北农学报,2026,41(1):49-55,7.基金项目
内蒙古自治区自然科学基金项目(2022MS03062) （2022MS03062）
