华北农学报2026，Vol.41Issue(1)：64-70,7.DOI:10.7668/hbnxb.20195933
大蒜水通道蛋白基因AsPIP1;1和AsTIP2;1的克隆、鉴定及其对盐胁迫的响应
Cloning and Characterization of Two Aquaporin Genes,AsPIP1;1 and AsTIP2;1,and Their Response to Salt Stress in Garlic
摘要
Abstract
Aquaporins(AQPs)are primarily responsible for the transmembrane transport of water molecules and are involved in processes such as plant growth,development,and responses to environmental stress.Garlic is an important vegetable crop widely cultivated worldwide,but there are currently few reports on salt-tolerance genes in garlic.It aimed to clone the genes AsPIP1;1 and AsTIP2;1,encoding plasma membrane intrinsic protein(PIP)and tonoplast intrinsic protein(TIP)respectively,from garlic,analyze their sequence characteristics,investigate their expression patterns under salt stress conditions,and evaluate their potential roles in garlic salt tolerance.We cloned the AsPIP1;1 and AsTIP2;1 genes from garlic using RT-PCR.Bioinformatics methods were employed to analyze the open reading frames,encode amino acid sequences,and conserve domains of the cloned genes.Furthermore,the expression profiles of AsPIP1;1 and AsTIP2;1 in different tissues and under salt stress conditions were detected u-sing Quantitative Real-time PCR.The results showed that the open reading frames of the AsPIP1;1 and AsTIP2;1 genes were 867,747 bp in length,encoding 288,248 amino acids,respectively,with the conserved NPA motifs.AsPIP1;1 and AsTIP2;1 were highly expressed in garlic leaves and roots,and salt stress strongly induced changes in the transcriptional levels of both genes.Furthermore,AsPIP1;1 and AsTIP2;1 may interact with other AQP pro-teins,cell wall-associated proteins,and transporter proteins to adapt to the salt stress environment.These results indicate that the AsPIP1;1 and AsTIP2;1 genes may be involved in the garlic plant's response to salt stress.关键词
大蒜/盐胁迫/水通道蛋白/表达分析/AsPIP1/1/AsTIP2/1Key words
Garlic/Salt stress/Aquaporins/Expression analysis/AsPIP1/1/AsTIP2/1分类
农业科技引用本文复制引用
吴嘉萁,许玉洁,陈洋洋,任旭琴,周瑾,熊爱生,王广龙..大蒜水通道蛋白基因AsPIP1;1和AsTIP2;1的克隆、鉴定及其对盐胁迫的响应[J].华北农学报,2026,41(1):64-70,7.基金项目
青海省科技厅重点实验室项目(2020-ZJ-Y02) （2020-ZJ-Y02）
青海省蔬菜遗传与生理重点实验室开放课题(2020-SCSYS-01) （2020-SCSYS-01）
淮阴工学院博士科研启动基金项目(Z301B16531) （Z301B16531）
