华北农学报2026，Vol.41Issue(1)：71-78,8.DOI:10.7668/hbnxb.20196164
向日葵苗期耐盐性状的全基因组关联分析
Genome-Wide Association Study of Salt Tolerance Traits in Sunflower at Seedling Stage
摘要
Abstract
Sunflower is an important oil crop with strong salt tolerance in China.However,the production of sunflower is severely restricted by the intensification of salinization in main producing areas.To understand the salt tolerance mechanism of salt tolerance-related traits at the seedling stage of sunflower,and to identify SNP loci and candidate genes significantly associated with salt tolerance at the seedling stage of sunflower,124 sunflower germ-plasm accessions were used as materials.Under the stress of 250 mmol/L NaCl,genome-wide association analysis and mining of salt-tolerance loci/genes were performed based on 5 traits including plant height,leaf area,aboveground fresh weight,underground fresh weight,and SPAD value.The results showed that after 14 days of salt stress,the five trait indexes of relative plant height,relative leaf area,relative fresh weight of the above-ground part,relative fresh weight of the underground part and relative SPAD value at seedling stage had significant variations and normal distributions,and the most obvious influences were the relative fresh weight of the underground part and rela-tive leaf area(with a coefficient of variation of over 46.57％).Eighteen significantly-associated SNP loci were ob-tained by genome-wide association analysis,among which Chr35448-18789497 was detected in several salt-tolerant traits;gene searches were conducted at 80 kb each in the upstream and downstream of associated loci,45 related candidate genes were screened out.Through scanning and gene annotation analysis of the associated locus intervals,four candidate genes that might be related to the salt tolerance trait of sunflower seedlings were discovered.A ge-nome-wide association analysis was conducted using the phenotypes of salt tolerance identification at the seedling stage and genomic SNP data for sunflower germplasm accessions.SNP loci significantly associated with salt tolerance at the seedling stage were detected,and candidate genes related to salt tolerance at the seedling stage of sunflower were selected,which laid the foundation for the subsequent verification of salt-tolerant genes.关键词
向日葵/种质资源/苗期耐盐性/全基因组关联分析/基因Key words
Sunflower/Germplasm resource/Salt tolerance at seedling stage/Genome-wide association study(GWAS)/Gene分类
农业科技引用本文复制引用
吴佳俊,李玉骁,张欣融,朱梓榕,谭美莲,汪磊,王玲,汪魏,张玉雪..向日葵苗期耐盐性状的全基因组关联分析[J].华北农学报,2026,41(1):71-78,8.基金项目
国家重点研发计划项目(2024YFD1600103) （2024YFD1600103）
国家特色油料产业技术体系(CARS-14-1-17) （CARS-14-1-17）
农业种质资源普查收集、保护鉴定服务项目 （）
