Abstract
Objective:To investigate the effect of alpinetin on inflammatory injury in nephrotic syndrome(NS)rats by regulating the Hippo/Yes-associated protein(YAP)signaling pathway.Methods:A NS model was established by injecting doxorubicin hydrochloride,and rats were randomly divided into NS group,different-dose alpinetin groups,prednisone group,alpinetin+Hippo/YAP inhibitor verteporfin(VP)group,and the control group which was injected with normal saline.The assay kit was used to detect the urine protein of rats;the biochemical analyzer was used to measure the serum creatinine(Scr)and blood urea nitrogen(BUN);the ELISA kit was used to detect the levels of monocyte chemoattractant protein-1(MCP-1),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6);both kidneys were collected,and the kidney index was measured;H-E staining was used to detect renal histopathological changes;the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL)method was used to detect apoptosis of renal tissue cells.Finally,real-time quantitative polymerase chain reaction(RT-qPCR)and Western blotting were employed to detect the mRNA and protein expressions of YAP and transcriptional co-activator PDZ-binding motif(TAZ).Results:Compared with the control group,the levels of Scr,urinary protein,BUN,IL-6,TNF-α,MCP-1,kidney index,pathological score,apoptosis rate,p-YAP/YAP,TAZ and mRNA expression in the NS group were significantly increased.Compared with the NS group,the levels of Scr,urinary protein,BUN,IL-6,TNF-α,MCP-1,kidney index,pathological score,apoptosis rate,p-YAP/YAP,TAZ mRNA and protein expression in the different-dose alpinetin groups and prednisone group were significantly reduced,and there were differences among different doses of alpinetin.Compared with the 160 mg/kg alpinetin group,the levels of Scr,urinary protein,BUN,IL-6,TNF-α,MCP-1,kidney index,pathological score,apoptosis rate,p-YAP/YAP,TAZ mRNA and protein expression in the alpinetin+VP group obviously reduced.Conclusion:Alpinetin has an effect on inflammatory injury in NS rats by regulating the Hippo/YAP signaling pathway.关键词
肾病综合征/山姜素/肾/Hippo/YAP信号通路/炎性损伤Key words
nephrotic syndrome/alpinetin/kidney/Hippo/YAP signaling pathway/inflammatory injury分类
医药卫生
