解剖学杂志2026，Vol.49Issue(1)：41-46,84,7.DOI:10.3969/j.issn.1001-1633.2026.01.008
原苏木素A调节AMPK/ACC信号通路对乳腺癌细胞增殖、凋亡和放射敏感性的影响
Impacts of protosappanin A on proliferation,apoptosis and radiosensitivity of breast cancer cells by modulating the AMPK/ACC signaling pathway
摘要
Abstract
Objective:To explore the impacts of protosappanin A(PrA)on the proliferation,apoptosis and radiosensitivity of breast cancer cells by modulating the AMP-activated protein kinase(AMPK)/acetyl-CoA carboxylase(ACC)signaling pathway.Methods:Breast cancer cells(MCF-7)were cultured in vitro and randomly divided into control group(normal culture),L-PrA group,M-PrA group,H-PrA group(treated with 400,800,1 600 μmol/L PrA),and Compound C group(treated with 1 600 μmol/L PrA and 1 μmol/L Compound C,an AMPK/ACC pathway inhibitor).Cell proliferation was assessed by CCK-8 assay to select appropriate PrA concentrations and to compare proliferative activity across groups.The colony formation experiment was performed to detect cell radiosensitivity.Scratch wound-healing and Transwell assays were adopted to measure cell migration and invasion.Flow cytometry was utilized to assess the apoptosis of cells in each group.Western blotting was employed to analyze the proteins associated with proliferation,migration,invasion,apoptosis,and the AMPK/ACC signaling pathway in cells.Results:Compared with the control group,PrA treatment groups showed dose-dependent decreases in cell survival fraction(SF),proliferation rate,migration rate,invasion number,and expression of PCNA,MMP-2,MMP-9,and Bcl-2,and increases in sensitization enhancement ratio(SER),apoptosis rate,and expression of Bax,cleaved caspase-3,p-AMPK/AMPK,and p-ACC/ACC.Compared with the H-PrA group,the Compound C group exhibited significant rises in SF,proliferation rate,migration rate,invasion number,and expression of PCNA,MMP-2,MMP-9,and Bcl-2,while demonstrating decreases in SER,apoptosis rate,and expression of Bax,cleaved caspase-3,p-AMPK/AMPK,and p-ACC/ACC.Conclusion:PrA may activate the AMPK/ACC signaling pathway,thereby inhibiting breast cancer cell proliferation,migration and invasion,promoting cell apoptosis,and enhancing radiosensitivity.关键词
乳腺癌/原苏木素A/AMP活化蛋白激酶/乙酰辅酶A羧化酶/增殖/凋亡/放射敏感性Key words
breast cancer/protosappanin A/AMP-activated protein kinase/acetyl-CoA carboxylase/proliferation/apoptosis/radiosensitivity分类
医药卫生引用本文复制引用
郭帅,徐雪峰,吴建亭..原苏木素A调节AMPK/ACC信号通路对乳腺癌细胞增殖、凋亡和放射敏感性的影响[J].解剖学杂志,2026,49(1):41-46,84,7.基金项目
南通市卫生健康委员会科研(MSZ2023033) （MSZ2023033）
