高师理科学刊2026，Vol.46Issue(2)：43-50,8.DOI:10.3969/j.issn.1007-9831.2026.02.008
橙皮素对脂多糖诱导人肠上皮细胞氧化损伤及凋亡的影响
Effect of hesperetin on oxidative damage and apoptosis of human intestinal epithelial cells induced by lipopolysaccharide
摘要
Abstract
Aimed to investigate the effects of Hesperetin(HPT)on oxidative damage and apoptosis in lipopolysaccharide(LPS)-induced human intestinal epithelial cells(NCM460).Cell viability of NCM460 cells was assessed using the MTT assay to determine the optimal concentration of HPT,and finally 50,200,400 μmol/L HPT were selected for subsequent experiments.The experimental groups included the control group,LPS group and different concentrations of HPT+LPS group.Biochemical assays were conducted to measure malondialdehyde(MDA)and reactive oxygen species(ROS)levels,glutathione peroxidase(GSH-Px)activity,superoxide dismutase(SOD)activity,total antioxidant capacity(T-AOC),as well as Caspase3/9 activity in the cells.The relative expression levels of antioxidant and apoptosis-related genes mRNA were determined using fluorescence quantitative PCR.The results showed that pretreatment with different concentrations of HPT significantly reduced MDA and ROS levels,increased GSH-Px,SOD activity,T-AOC and decreased Caspase3/9 activity compared to the LPS group.The relative expression levels of antioxidant-related genes Nrf2,HO-1,NQO1,SOD1 and anti-apoptotic factor Bcl-2 mRNA were significantly upregulated,while pro-apoptotic factors Caspase3/9 and Bax mRNA were significantly downregulated.These findings indicate that appropriate concentrations of HPT can effectively inhibit oxidative damage and apoptosis in LPS-induced NCM460 cells.关键词
橙皮素/脂多糖/人肠上皮细胞/氧化损伤/细胞凋亡Key words
hesperetin/lipopolysaccharide/human intestinal epithelial cells/oxidative damage/apoptosis分类
生物科学引用本文复制引用
杨佳欣,王鑫宇,刘柏琦,王雨鑫,杨紫琪,黄鑫..橙皮素对脂多糖诱导人肠上皮细胞氧化损伤及凋亡的影响[J].高师理科学刊,2026,46(2):43-50,8.基金项目
黑龙江省省属高等学校基本科研业务费科研项目(145409451) （145409451）
黑龙江省大学生创新创业训练计划项目(S202510232105) （S202510232105）
