高师理科学刊2026，Vol.46Issue(2)：57-66,98,11.DOI:10.3969/j.issn.1007-9831.2026.02.010
砂藓RcPM19L1基因的耐旱和耐高温功能
Drought and high-temperature tolerance functions of the RcPM19L1 in Racomitrium canescens
摘要
Abstract
The ABA-induced wheat plasma membrane polypeptide-19 protein(AWPM-19)is closely related to plant tolerance to abiotic stresses such as drought and high-temperature.Racomitrium canescens can quickly recover to its normal growth state upon rehydration after maintaining a relative water content under 10%for many years,and it also exhibits strong high-temperature tolerance.For analyzing the drought and high-temperature tolerance function of the AWPM-19 family gene RcPM19L1,which is in response to dehydration stress in R.canescens,exploring whether RcPM19L1 can enhance the drought and high-temperature tolerance characteristics of plants,providing a theoretical basis for further studying the role of AWPM-19 family genes in regulating plant stress resistance.The expression of RcPM19L1 under different treatments of dehydration and high temperature stress in R.canescens was detected using real-time quantitative PCR.The coding sequence of RcPM19L1 was cloned,transgenic RcPM19L1 overexpression Arabidopsis thaliana lines were acquired,the phenotype under drought and high-temperature stress was observed,and physiological and biochemical indexes were analyzed.RcPM19L1 is capable of responding to dehydration and high-temperature stress in R.canescens.The full-length coding sequence of RcPM19L1 is 525 bp,encoding a protein containing 174 amino acid residues,and is predicted to possess the characteristic structures of AWPM-19.The RcPM19L1 transgenic overexpression A.thaliana lines was successfully constructed.It was found there was no significant difference in the growth status between wild-type A.thaliana and transgenic plants under drought treatment.However,under high-temperature treatment,the phenotype of RcPM19L1 transgenic A.thaliana was superior to that of wild-type plants.Under high temperature stress,compared to wild-type A thaliana,in RcPM19L1 transgenic plants,the mass fraction of some osmotic adjustment substances increased,the peroxidase activity can reach up to approximately 1.75 times of the wild-type plants,and the catalase activity can reach up to approximately 1.22 times of the wild-type plants.Heterologous overexpression of RcPM19L1 in A.thaliana improves the high-temperature tolerance,but no significantly changes on the drought tolerance.关键词
RcPM19L1/砂藓/耐高温性/耐旱性Key words
RcPM19L1/Racomitrium canescens/high-temperature tolerance/drought tolerance分类
农业科技引用本文复制引用
张晨旭,鞠艳,蔡璨,乔梦雪,谷新颖,沙伟,张梅娟,彭疑芳,马天意..砂藓RcPM19L1基因的耐旱和耐高温功能[J].高师理科学刊,2026,46(2):57-66,98,11.基金项目
黑龙江省省属高等学校基本科研业务费科研项目(145409452) （145409452）
