菌物研究2026，Vol.24Issue(1)：79-86,8.DOI:10.13341/j.jfr.2024.1766
玉木耳多糖热水提取工艺优化及抗氧化活性研究
Optimization of Hot Water Extraction Techniques and Evaluation of Antioxidant Activity of Polysaccharides Derived from Auricularia cornea'Yumuer'
摘要
Abstract
This study employed hot water extraction to isolate polysaccharides from Auricularia cornea'Yumuer'(ACP),examining the effects of extraction temperature,number of extraction cycles,liquid-to-solid ratio,and extraction duration on ACP yield.Both single-factor experiments and response surface methodology were utilized to optimize the extraction parameters and identify the optimal conditions for ACP recovery.The antioxidant activity of ACP was assessed through free radical scavenging assays.Results indicated that the optimal extraction conditions were an extraction temperature of 100℃,extraction time of 251 minutes,three extraction cycles,and a liquid-to-solid ratio of 71∶1(mL∶g).Under these conditions,the ACP yield reached 10.07％,with a deviation of 0.7％from the predicted value.The half-maximal inhibitory concentration(IC50)values for scavenging DPPH,ABTS,hydroxyl,and superoxide anion radicals were 1.87,1.13,2.19,and 0.94 mg/mL,respectively,demonstrating dose-dependent antioxidant activity.These findings suggest that ACP possesses significant antioxidant properties,providing a scientific foundation for its further development,utilization,and potential economic value enhancement.关键词
玉木耳多糖/热水提取/单因素试验/响应面分析/自由基清除Key words
Auricularia cornea'Yumuer'polysaccharide/hot water extraction/single factor test/response surface analysis/radical-scavenging分类
农业科技引用本文复制引用
刘亚青,王天赐,李顺玲,苏玲..玉木耳多糖热水提取工艺优化及抗氧化活性研究[J].菌物研究,2026,24(1):79-86,8.基金项目
现代农业产业技术体系项目(CARS-20-17),吉林省自然科学基金项目(20220101339JC) （CARS-20-17）
