生物灾害科学2026，Vol.49Issue(1)：60-68,9.DOI:10.3969/j.issn.2095-3704.2026.01.08
黄龙病对赣南脐橙内生细菌和根际土壤细菌群落多样性影响的研究
Study on the effects of citrus Huanglongbing on the diversity of endophytic bacteria and rhizosphere soil bacterial communities in Gannan Navel Oranges
摘要
Abstract
[Objective]To investigate the diversity and community composition of endophytic bacteria and rhizosphere soil bacterial microorganisms in Gannan Navel Oranges infected with Huanglong disease and healthy Gannan Navel Oranges,and to explore the changes in species composition and community structure of endophytic bacteria and rhizosphere soil bacterial microorganisms in Gannan Navel Oranges under disease and non disease conditions.[Method]Taking Gannan Navel Orange as the research object,leaf,fruit,root,and rhizosphere soil samples of Huanglongbing and non diseased plants were collected for 16S rDNA amplicon sequencing.High throughput sequencing technology was used to obtain various information such as bacterial species composition,community abundance,and community differences comparison in specific experimental samples.[Result]Among healthy and diseased navel oranges,the OTUs of rhizosphere soil bacteria were the highest,followed by roots and leaves,and the lowest in fruits;The Shannon index values of endophytic bacteria and rhizosphere soil bacteria in healthy navel orange fruits,leaves,and roots were higher than those in susceptible samples,indicating that the species diversity of endophytic bacteria and rhizosphere soil bacteria in healthy navel orange fruits,leaves,and roots was higher than that in susceptible navel oranges;The endophytic bacteria in healthy and diseased navel oranges mainly include Cyanobacteria at the phylum level,with the lowest relative abundance of Cyanobacteria in roots and the highest relative abundance of Cyanobacteria in leaves;Among the bacteria in the rhizosphere soil of healthy and diseased navel oranges,Pseudomonas,Actinomycota,Acidobacteriota,and Chloroflexita are the main dominant bacterial phyla.[Conclusion]The diversity of endophytic bacteria and rhizosphere soil bacterial communities in susceptible and healthy leaves,fruits,roots,and rhizosphere soil of Gannan Navel Oranges is abundant,and there are related beneficial bacteria.This provides new reference for exploring the utilization pathways of endophytic bacteria and rhizosphere soil bacterial communities in Gannan Navel Oranges in the future,as well as green prevention and control research on citrus huanglongbing.关键词
赣南脐橙/柑橘黄龙病/内生细菌群落多样性/根际土壤/高通量测序Key words
Gannan Navel Orange/citrus Huanglongbing/diversity of endophytic bacteria/rhizosphere soil/high throughput sequencing分类
农业科技引用本文复制引用
凌长有,姜晨,龚武滨..黄龙病对赣南脐橙内生细菌和根际土壤细菌群落多样性影响的研究[J].生物灾害科学,2026,49(1):60-68,9.基金项目
2024年度江西省农牧渔业科研指导性课题 （）
