棉花学报2025，Vol.37Issue(6)：465-477,13.DOI:10.11963/cs20250057
棉纤维黑孢霉腐烂病的病原鉴定及其发生与螨类的关系
Identification of the pathogen causing cotton Nigrospora lint rot and the interdependence between the fungus and mite
摘要
Abstract
[Objective]A disease that damages cotton fiber has been found in the Xinjiang cotton fields in recent years.The infected cotton fiber turned into mouse-gray and failed to fluff as the boll opens,losing all commercial value.The purpose of this study was to identify the pathogen causing lint rot in Xinjiang cotton fields,and to explore the relationship between mite and disease transmission.[Methods]A total of 161 diseased boll samples were collected from 25 cotton-growing units across northern and southern Xinjiang in 2021 and 2023.The strains were isolated and purified using the tissue isolation method.The pathogen was identified based on morphological characteristics,molecular biological analysis,and pathogenicity test.Different tissues and organs of cotton plants were artificially inoculated in the field to determine the infection sites of the pathogen.The correlation between cotton boll rot and mite was investigated by examining the occurrence frequency of the mite in diseased bolls in cotton field,and combining with the experiment of artificially inoculating healthy detached cotton bolls with mites carrying conidia.[Results]The cotton Nigrospora lint rot is widespread in Xinjiang cotton fields,and the damage is gradually worsening.A total of 146 Nigrospora strains were isolated from diseased samples collected from cotton fields in Xinjiang.The morphological characteristics of the strains were consistent with N.gorlenkoana.The DNA sequences of the internal transcribed spacer in eukaryotic ribose rDNA-ITS,translation elongation factor TEF1-α,and β-tubulin TUB2 in 27 representative strains exhibited high homology with N.gorlenkoana.Field and laboratory pathogenicity tests of 5 representative strains showed that the lint displayed extensive black-gray rot.Therefore,the pathogen causing cotton Nigrospora lint rot in Xinjiang was identified as N.gorlenkoana.The pathogen exclusively infected cotton fiber and did not affect seed,leaf,stem,bud,and flower of cotton.Siteroptes reniformis was closely associated with cotton Nigrospora lint rot.Adult mites or their physogastric females were found in all the diseased bolls,and they can carry the conidia of N.gorlenkoana into the bolls to spread the disease.[Conclusion]The cotton lint rot caused by N.gorlenkoana in Xinjiang in recent years is a disease specifically targeting cotton fiber,with S.reniformis as the primary transmission vector.关键词
棉纤维腐烂病/Nigrospora gorlenkoana/病原菌鉴定/肾形穗螨/传播介体Key words
cotton lint rot/Nigrospora gorlenkoana/pathogen identification/Siteroptes reniformis/transmission vector引用本文复制引用
马智玉,李国英,任毓忠,刘锦华,苏杰,杜娟,张建萍,刘升学..棉纤维黑孢霉腐烂病的病原鉴定及其发生与螨类的关系[J].棉花学报,2025,37(6):465-477,13.基金项目
国家自然科学基金(32560634) （32560634）
中华人民共和国科学技术部中小企业创新项目(14C26216513812) （14C26216513812）
