棉花学报2025，Vol.37Issue(6)：478-489,12.DOI:10.11963/cs20250047
GhSWEET55在棉花抗黄萎病菌中的功能分析
Functional analysis of GhSWEET55in cotton resistance to Verticillium dahliae
摘要
Abstract
[Objective]This study aimed to investigate the role of sucrose in cotton resistance against Verticillium wilt and verify the function of GhSWEET55,providing a theoretical basis for revealing the molecular mechanism of cotton resistance to Verticillium wilt.[Methods]Cotton were inoculated with Verticillium dahliae using the root-wounding method.Sucrose content in cotton roots was determined by spectrophotometry.Differentially expressed genes were obtained by transcriptome sequencing.The enrichment of differentially expressed genes was studied by KEGG pathway analysis.Differentially expressed genes and SWEET family expression patterns were analyzed by transcriptome sequencing,and validated by quantitative real-time polymerase chain reaction(qRT-PCR).Bioinformatics analysis was performed to characterize the protein sequence features and phylogenetic relationships of GhSWEET55,and its expression pattern was analyzed by qRT-PCR.The function of GhSWEET55 was verified by virus-induced gene silencing(VIGS).[Results]Sucrose content in cotton roots increased significantly at 12 h and 48 h after V.dahliaeinfection,and decreased significantly at 96 h after V.dahliae infection.Some differentially expressed genes were enriched in sucrose-related energy metabolism pathways and defense response pathways.Most members of the SWEET family were differentially expressed,and GhSWEET55 from Clade Ⅲ was significantly up-regulated.GhSWEET55 was predominantly expressed in cotton leaves,and the encoded protein was localized to the plasma membrane.Silencing of GhSWEET55 enhanced cotton resistance to Verticillium wilt.[Conclusion]Sucrose is involved in cotton resistance against Verticillium wilt.The SWEET gene family responds to V.dahliae infection.GhSWEET55 negatively regulates cotton resistance to V.dahliae.关键词
黄萎病/转录组/蔗糖/SWEET基因家族Key words
Verticillium wilt/transcriptome/sucrose/SWEET gene family引用本文复制引用
杨莹莹,杨代刚..GhSWEET55在棉花抗黄萎病菌中的功能分析[J].棉花学报,2025,37(6):478-489,12.基金项目
新疆生产建设兵团科技创新工程(2024AB002) （2024AB002）
国家南繁专项(YBXM2549) （YBXM2549）
国家棉花产业技术体系(CARS-15-08) （CARS-15-08）
