棉花学报2025，Vol.37Issue(6)：490-500,11.DOI:10.11963/cs20250032
棉花FOXG1基因家族的鉴定及功能初探
Identification and preliminary functional analysis of the FOXG1 gene family in cotton
摘要
Abstract
[Objective]Forkhead box protein G1(FOXG1),as a transcription factor,plays a crucial role in cell proliferation and differentiation.At present,the FOXG1 gene family has not been systematically studied in cotton.This research aimed to identify and analyze FOXG1 gene family in cotton.[Methods]FOXG1 gene family members were identified in cotton using bioinformatic approaches.Analyses were performed on the physicochemical properties,conserved motifs,and phylogenetic relationships of the corresponding proteins.The expression patterns of FOXG1 genes in Gossypium hirsutum and G.barbadense were investigated using transcriptome data.Preliminary functional exploration of the FOXG1 genes was conducted through association analysis between haplotypes of G.hirsutum FOXG1 genes and fiber quality traits,as well as via transgenic overexpression of GhFOXG1-4 gene in Arabidopsis thaliana.[Results]A total of 6,5,3,3,6,6,6,and 3 FOXG1 genes were identified in G.hirsutum,G.barbadense,G.raimondii,G.arboreum,G.tomentosum,G.mustelinum,G.darwinii,and G.herbaceum,respectively.These 38 FOXG1 proteins and 3 FOXG1 proteins of A.thaliana could be divided into 4 clades.GhFOXG1-1 was highly expressed in anther and ovule.GhFOXG1-2,GbFOXG1-1 and GbFOXG1-2 were highly expressed in anther,ovule and fiber.GhFOXG1-4 was highly expressed in ovule.GhFOXG1-4 and GhFOXG1-5 had 2 and 3 haplotypes,respectively.Significant differences in upper half mean length,breaking strength,and micronaire were observed among these haplotypes.The stem cell width of A.thalianaoverexpressing GhFOXG1-4gene was significantly larger than that of wild type.[Conclusion]A total of 38 FOXG1 genes were identified in 8 cotton species.The excellent haplotypes of some GhFOXG1 genes have the potential to synergistically improve fiber quality.GhFOXG1-4 positively regulates the stem cell width in A.thaliana,which lays a foundation for further study the function of FOXG1 family genes.关键词
棉花/FOXG1基因家族/生物信息学/表达分析/GhFOXG1-4/单倍型Key words
cotton/FOXG1 gene family/bioinformatics/expression analysis/GhFOXG1-4/haplotype引用本文复制引用
周曹屹,郑凯,王莉,赛热比艳·阿布都加帕尔,王刚,陈全家,裴文锋,张滋林,宋吉坤,张兵兵,贾冰,冯盼,吴嫚,于霁雯..棉花FOXG1基因家族的鉴定及功能初探[J].棉花学报,2025,37(6):490-500,11.基金项目
国家自然科学基金(32301808) （32301808）
新疆维吾尔自治区自然科学基金(2024D01A131) （2024D01A131）
国家重点研发计划(2022YFD1200300) （2022YFD1200300）
河南省农业良种联合攻关项目(2022010301) （2022010301）
