棉花学报2025，Vol.37Issue(6)：525-535,11.DOI:10.11963/cs20250033
海岛棉酵母双杂交文库的构建及GbbHLH130互作蛋白的筛选
Construction of a yeast two-hybrid library for Gossypium barbadense(sea island cotton)and screening of GbbHLH130-interacting proteins
摘要
Abstract
[Objective]Construct a yeast two-hybrid(Y2H)library of Gossypium barbadense,and screen for interacting proteins of GbbHLH130 using Y2H assays.[Methods]This study constructed a Y2H library of G.barbadense using the Gateway technique,and employed the Y2H system to screen for candidate proteins interacting with GbbHLH130.[Results]This study successfully constructed a G.barbadense Y2H library,with a second library capacity of 1.36×107 CFU,and insert fragment length>1 000 bp,and a recombination rate of 100%.Using Y2H technology,298 initial positive clones were screened,and the interaction between GbRAV1,GbHSFA1b,and GbbHLH130 was verified through Y2H assays.[Conclusion]This study successfully constructed a Y2H library of G.barbadense and preliminarily screened candidate interacting proteins for GbbHLH130.GbRAV1 and GbHSFA1b were verified to interact with GbbHLH130 through Y2H assays,suggesting that they may play a role in the biological process of response to drought stress mediated by GbbHLH130.However,their interaction mechanisms and specific roles in drought response need further research.关键词
海岛棉/GbbHLH130/酵母双杂交文库/干旱胁迫Key words
island cotton/GbbHLH130/yeast two-hybrid library/drought stress引用本文复制引用
王嘉惠,陈俊琛,于月华,倪志勇,王怡..海岛棉酵母双杂交文库的构建及GbbHLH130互作蛋白的筛选[J].棉花学报,2025,37(6):525-535,11.基金项目
新疆维吾尔自治区自然科学基金(2023D01B30) （2023D01B30）
新疆维吾尔自治区人才发展基金"天池英才"引进计划项目 （）
2022年新疆维吾尔自治区"天山英才"培养计划青年科技拔尖人才-青年科技创新人才(2022TSYCCX0080) （2022TSYCCX0080）
中国博士后科学基金第73批面上资助"地区专项支持计划"(2023MD734233) （2023MD734233）
