北方农业学报2026，Vol.54Issue(1)：43-55,13.DOI:10.12190/j.issn.2096-1197.2026.01.05
桔梗bHLH基因家族鉴定与表达分析
Identification and expression analysis of the bHLH gene family of Platycodon grandiflorus
摘要
Abstract
[Objective]To investigate the characteristics and functions of the bHLH gene family of Platycodon grandiflorus.[Methods]Analyses of physicochemical properties,phylogenetic tree,conserved motifs,gene structures,cis-regulatory elements,and collinearity analysis were conducted for the bHLH gene family of P.grandiflorus.Quantitative real-time PCR(qRT-PCR)was used to examine the expression patterns of bHLH genes in tissues of P.grandiflorus.[Results]A total of 94 members were identified in the bHLH gene family of P.grandiflorus,which were classified into nine subfamilies and distributed across eight chromosomes.Conserved motif analysis showed that Motif1 and Motif2 were the major conserved motifs of this family.The gene structures were diverse,containing 1-11 exons.Analysis of cis-regulatory elements in the promoters revealed that they contained response elements associated with light responsiveness,plant hormones,and stress.Collinearity analysis identified 18 pairs of duplicated bHLH genes.Tissue-specific expression analysis indicated that PgbHLH65,PgbHLH8,and PgbHLH72 were higher relative expression in stems,PgbHLH82 and PgbHLH20 showed higher relative expression in roots,and PgbHLH10,PgbHLH52,and PgbHLH91 exhibited higher relative expression in leaves.[Conclusion]Different bHLH genes may perform specific functions in the roots,stems,and leaves of P.grandiflorus.关键词
桔梗/bHLH基因/转录因子/生物信息学/组织表达模式Key words
Platycodon grandiflorus/bHLH gene/Transcription factor/Bioinformatics/Tissue expression pattern分类
农业科技引用本文复制引用
陈子怡,汪晓蕊,何涵,李子墨,夏自玉,黄文静,孙晓春..桔梗bHLH基因家族鉴定与表达分析[J].北方农业学报,2026,54(1):43-55,13.基金项目
国家自然科学基金青年科学基金项目(81703656) （81703656）
陕西省科技厅自然科学基础研究项目(S2025-JC-YB-0636) （S2025-JC-YB-0636）
陕西省教育厅项目(23JY026) （23JY026）
