江西农业学报2026，Vol.38Issue(3)：41-50,10.DOI:10.19386/j.cnki.jxnyxb.2026.03.006
珍稀蕨类金毛狗脊原生质体培养研究
Study on Protoplast Culture of Rare Fern Cibotium barometz
摘要
Abstract
To establish an efficient and stable protoplast culture system for Cibotium barometz,this study used Cibotium barometz spores as explants to systematically investigate the effects of different medium combinations on sporophyte formation.The changes in physiological and biochemical characteristics of prothallia and sporophytes at different culture stages were analyzed,and suitable materials for protoplast culture were screened for further protoplast culture studies of Cibotium barometz sporophytes.The results showed that the optimal medium for sporophyte formation was MS+0.1 mg/L NAA+0.2 mg/L KT.The TP content,as well as the activities of POD and SOD,were significantly higher in sporophytes than in prothalli.Using sporophyte protoplasts as the culture material,the best culture results were achieved in a shallow liquid medium of KM8P+1.0 mg/L 6-BA+0.5 mg/L 2,4-D+6.0%glucose+2.0%sucrose,with a density of 1×105 cells/mL.Under these conditions,the first division of protoplasts occurred at 2 days,small cell clusters were observed at 14 days,and callus tissues of approximately 2 mm in size developed after 28 days.The callus tissues could continuously proliferate on MS solid medium.This study can provide key technical parameters for the protoplast culture and plant regeneration system of Cibotium barometz.关键词
金毛狗脊/孢子体/原生质体分离/原生质体培养Key words
Cibotium barometz/Sporophyte/Protoplast isolation/Protoplast culture分类
农业科技引用本文复制引用
杨帆,丁妹,杜勤..珍稀蕨类金毛狗脊原生质体培养研究[J].江西农业学报,2026,38(3):41-50,10.基金项目
2019第四次全国中药资源普查项目(GZYKJS-2019-003) （GZYKJS-2019-003）
广东省科技特派员项目(KTP20200137) （KTP20200137）
广州市重点研发和社会发展科技计划项目(2023B03J1273). （2023B03J1273）
