生态学杂志2026，Vol.45Issue(2)：538-543,6.DOI:10.13292/j.1000-4890.202602.030
杉木人工林土壤真菌反硝化功能基因丰度特征及其环境影响因子
Characteristics and environmental driving factors of fungal denitrification functional gene abundance in soils of Chinese fir plantation in Fujian Province
摘要
Abstract
Subtropical forest soil is a hot spot for fungal denitrification,which is an important pathway for the pro-duction of nitrous oxide(N2O)in soils.However,the distribution pattern of the denitrifying fungal functional gene(FnirK)and its environmental drivers in subtropical Chinese fir plantations remain unclear.In this study,a total of 108 soil samples were collected from nine Chinese fir plantations in Fujian Province,and the abundance of FnirK gene was measured using real-time quantitative PCR.The results showed that the physicochemical properties and the abundance of FnirK gene exhibited significant spatial heterogeneity.The FnirK gene abundance in Guanzhuang State-owned Forest Farm,Shaxian County,Sanming City was the highest,at 19.75×108 copies·g-1,while its abundance in Wuyi State-owned Forest Farm,Zhangping City,Longyan City was the lowest,at 3.94×108 copies·g-1.The average abundance of FnirK gene in the nine plantations was 11.9×108 copies·g-1.Random forest analy-sis indicated that soil pH was the most important environmental factor affecting FnirK gene abundance,explaining 21.02％of the total variations.FnirK gene abundance was significantly positively correlated with soil pH and mean annual precipitation(MAP),and significantly negatively correlated with soil dissolved organic carbon(DOC)and total carbon(TC).In conclusion,there is significant spatial heterogeneity in the abundance of denitrifying fungal functional gene FnirK in the soils of Chinese fir plantations in Fujian Province,with soil pH,DOC content,and MAP being the main factors influencing its distribution.关键词
真菌反硝化/FnirK基因/亚热带森林/氧化亚氮Key words
fungal denitrification/FnirK gene/subtropical forest/nitrous oxide引用本文复制引用
陈琦润,林欣宏,邓米林,杨浩,张晗烁,郑勇,林永新..杉木人工林土壤真菌反硝化功能基因丰度特征及其环境影响因子[J].生态学杂志,2026,45(2):538-543,6.基金项目
国家自然科学基金项目(42377301)资助. （42377301）
