现代检验医学杂志2026，Vol.41Issue(2)：141-145,5.DOI:10.3969/j.issn.1671-7414.2026.02.024
缺氧缺血性脑病新生儿血清NF-L、AQP9、FGL2水平表达与NABA评分的相关性研究
Correlation Study Between Serum NF-L,AQP9 and FGL2 Levels and NABA Scores in Neonatal Hypoxic-Ischemic Encephalopathy
摘要
Abstract
Objective To investigate the changes in serum levels of neurofilament light chain(NF-L),aquaporin 9(AQP9),and fi-brinogen-like protein 2(FGL2)in neonatal hypoxic-ischemic encephalopathy(HIE),and to analyze the correlation between the three markers and the neonatal behavioral neurological assessment(NABA)scores in affected infants.Methods From January 2022 to July 2024,203 infants with HIE admitted to Shanxi Children's Hospital were enrolled as the patient group.According to disease se-verity,they were assigned into mild group(63 cases),moderate group(81 cases)and severe group(59 cases).An additional 203 healthy neonates from the same period were selected as the control group.Serum NF-L,AQP9 and FGL2 levels were measured by ELISA.General clinical data were collected and analyzed.Receiver operating characteristic(ROC)curves were plotted to analyze the diagnostic value of NF-L,AQP9,FGL2 and NABA scores for severe HIE in neonates.Pearson correlation analysis method was used to assessed the correlation between serum NF-L,AQP9,FGL2 and NABA scores.Results Compared with the control group,the patient group exhibited significantly higher serum levels of serum NF-L(14.78±1.67 ng/ml vs 10.65±1.21 ng/ml),AQP9(61.82±6.29 pg/ml vs 42.62±4.31 pg/ml)and FGL2(189.69±20.26 ng/ml vs 132.51±14.84 ng/ml)(t=28.533,35.877,32.440,all P＜0.05).Serum levels of NF-L(14.68±1.67 ng/ml,18.62±2.03 ng/ml),AQP9(63.51±6.44 pg/ml,69.63±7.03 pg/ml)and FGL2(195.68±20.84 ng/ml,210.65±22.36 ng/ml)were significantly higher in the severe and moderate groups than those in the mild groups(11.32±1.33 ng/ml,52.32±5.41 pg/ml,162.35±17.55 ng/ml),while the NABA score(34.89±3.51,29.88±3.43)was significantly lower than that in the mild groups(36.54±1.52)(tmoderate group=4.616～16.729,tsevere group=17.276～33.701,all P＜0.05).NF-L,AQP9 and FGL2 levels were all negatively correlated with NABA scores(r=-0.432,-0.421,-0.402,all P＜0.05).The AUC for combined diagnosis of severe disease using NF-L,AQP9,FGL2 and NABA was significantly higher than that for individual markers(Z=2.066～3.307,all P＜0.05).Conclusions Serum levels of NF-L,AQP9,and FGL2 in neonates with HIE are greatly ele-vated and correlated with NABA score,potentially serving as biomarkers for assessing HIE severity.关键词
新生儿缺氧缺血性脑病/神经纤维轻链/水通道蛋白9/纤维蛋白原样蛋白2/新生儿行为神经评估Key words
neonatal hypoxic-ischemic encephalopathy/neurofilament light chain/aquaporin 9/fibrinogen-like protein 2/neonatal behavioral neurological assessment分类
医药卫生引用本文复制引用
李海霞,冀湧,王荣华..缺氧缺血性脑病新生儿血清NF-L、AQP9、FGL2水平表达与NABA评分的相关性研究[J].现代检验医学杂志,2026,41(2):141-145,5.基金项目
山西省卫生健康委员会科研课题项目(2022A117). （2022A117）
