实验科学与技术2026，Vol.24Issue(2)：34-41,8.DOI:10.12179/1672-4550.20240441
Co/NC纳米酶检测葡萄糖综合实验设计
Comprehensive Experimental Design for Glucose Detection by Co/NC Nanozymes
摘要
Abstract
In this paper,a comprehensive experiment is designed to synthesize Co/NC nanozymes derived from metal-organic frameworks for the detection of glucose content.A series of instruments are used to characterize the Co/NC nanozymes.Through control variable method,kinetic tests,and standard curve method,the testing conditions of nanozymes and the detection range for glucose are optimized.The experimental results show that when the molar ratio of Co to Zn is 1:1,the carbonization temperature is 900℃,and pH is 3.5,the Co/NC nanozymes exhibit the greatest oxidase-like activity.The maximum reaction rate of Co/NC nanozymes is 1.74×10-7 mol·L-1·s-1,and the nanozymes demonstrate a strong affinity for the substrate TMB.The linear range for glucose detection is 1～60 μmol,and the detection system exhibits excellent selectivity.The experiment integrates multidisciplinary knowledge,helping students achieve a comprehensive understanding of the knowledge system,develop scientific research thinking,improve innovation ability,and cultivate scientific literacy.关键词
金属有机骨架化合物/纳米酶/酶活性/葡萄糖检测/综合实验设计Key words
metal-organic frameworks/nanozymes/enzyme activity/glucose detection/comprehensive experimental design分类
化学化工引用本文复制引用
马昕雅,江泽群,陆海嘉,郑景文,闵喜军,徐丹..Co/NC纳米酶检测葡萄糖综合实验设计[J].实验科学与技术,2026,24(2):34-41,8.基金项目
海南医学院校级教育科研课题(HYYB202160) （HYYB202160）
海南省自然科学基金(222RC681) （222RC681）
海南医学院大学生创新创业研究项目(X202211810136). （X202211810136）
