现代检验医学杂志2026，Vol.41Issue(2)：192-197,6.DOI:10.3969/j.issn.1671-7414.2026.02.032
CRISPR-Cas12a系统在病原体检测领域的最新研究进展
Recent Advances in the CRISPR-Cas12a System for Pathogen Detection
摘要
Abstract
The clustered regulatory interspaced short palindromic repeat-associated protein 12a(CRISPR-Cas12a)system is a natural immune defense mechanism derived from bacteria,which has garnered significant attention in the field of nucleic acid analysis due to its high specificity,high sensitivity,equipment-free operation,simplicity and remarkable cost-effectiveness.The CRISPR-Cas12a system can be integrated with related technologies such as amplification techniques,biosensors,Raman spec-troscopy,and microfluidics to enhance the detection sensitivity of the system,reduce cross contamination,shorten the testing time,and broaden the application scenarios.In recent years,the CRISPR-Cas12a technology has been widely applied in nucleic acid detection for pathogens such as viruses,bacteria and mycoplasmas.This article primarily reviews the research advances of the CRISPR-Cas12a system in the aforementioned pathogen detection applications,provides an in-depth analysis of its advantag-es and limitations,and proposes practical strategies to deepen its implementation and iterative refinement in rapid pathogen iden-tification,precise diagnosis,and on-site screening scenarios.关键词
成簇规律的间隔短回文重复序列-关联蛋白12a系统/病原体/核酸检测Key words
clustered regulatory interspaced short palindromic repeat-associated protein 12a system/pathogen/nucleic acid testing分类
医药卫生引用本文复制引用
马贤参,常新昊,司文喆..CRISPR-Cas12a系统在病原体检测领域的最新研究进展[J].现代检验医学杂志,2026,41(2):192-197,6.基金项目
北京市科技新星计划(20220484090) （20220484090）
北京市科技新星交叉合作课题(20230484442) （20230484442）
北京市自然科学基金(7232206) （7232206）
血管稳态与重构全国重点实验室研究基金资助(2024-VHR-SY-13). （2024-VHR-SY-13）
