土壤学报2026，Vol.63Issue(1)：230-240,11.DOI:10.11766/trxb202501240041
耐盐藻菌的筛选及其联合体在盐渍化土壤改良中的作用研究
Screening of Salt-tolerant Algae and Bacteria and the Role of Theirs Consortium in Salinized Soil Improvement
摘要
Abstract
[Objective]Soil salinization can not only lead to the deterioration of soil physical and chemical properties,but also can seriously harm the growth and development of crops,causing severe harm to agricultural production and the ecological environment.Using microorganisms to improve the quality of salinized soil is a clean and efficient way,but there are also problems,such as the single function of microorganisms and the limited improvement effect.Nevertheless,and algae-bacterial consortium can fully exert the synergistic effect of algae and bacteria and enhance the effect of algae and bacteria on soil.However,at present,there are few related studies on the improvement of salinized soil using the algae-bacterial consortium.[Method]In this study,five microalgae and ten bacteria were isolated from salinized soil.After comparing the changes of biomass and specific growth rate in algae or bacteria under different salt stress,three salt-tolerant algae and four salt-tolerant bacteria were screened out.Afterwards,the three salt-tolerant algae and four salt-tolerant bacteria were combined into different algae-bacterial consortia.By comparing the changes in photosynthetic pigment content of algae in each algae-bacterial consortium,three salt-tolerant algae-bacterial consortia were further selected.Subsequently,the changes in EPS secretion and desalination effects were analyzed under different degrees of salt stress,and the optimal consortium of algae-bacteria was identified.Moreover,18S-rDNA sequencing or 16S-rDNA sequencing was performed on algae-1 and bacteria-8,and the phylogenetic trees were constructed using the neighbor-joining method,respectively.Finally,the algae1-bacteria8 consortium was applied to salinized soils to improve soil quality.[Result]According to the constructed phylogenetic tree,algae-1 was identified as Borodinellopsis sp.,and bacteria-8 belonged to Bacillus sp.After 10-day culture,the exopolysaccharide(EPS)secretion of algae and bacteria increased significantly by 44.56%and 43.19%at treatments of 15 g·L-1 and 20 g·L-1 salt stress,respectively.The soluble salt content of the solution decreased significantly,and the salt removal rate reached 57.89%and 57.55%at treatments of 15 g·L-1 and 20 g·L-1,respectively.When Borodinellopsis,Bacillus,and algae-bacterial consortium were respectively inoculated into salinized soil surface for 30 days,EPS content in soil was significantly increased by 51.72%,8.20%and 185.88%,and the salinity of soil leaching solution decreased by 5.10%,3.45%and 7.00%,respectively.Moreover,the salinity of the soil leaching solution inoculated with an algae-bacterial consortium was significantly lower than that of the soil inoculated with algae or bacteria alone.Concurrently,the total nitrogen content of soil in algae-bacterial consortium was significantly increased by 55.33%compared with the initial group,while no significant changes were observed in treatments of algae or bacteria alone.[Conclusion]Collectively,although both soil microalgae and bacteria can reduce soil salinity,algae-bacterial consortium have better effects on salinized soil than algae or bacteria alone.This study will provide an important theoretical basis for improving salinized soil with an algae-bacterial consortium.关键词
盐渍化/藻菌联合/土壤改良/盐胁迫Key words
Salinization/Algae-bacteria coordination/Soil improvement/Salt stress分类
资源环境引用本文复制引用
舒雪,赵晨宇,施军琼,吴忠兴..耐盐藻菌的筛选及其联合体在盐渍化土壤改良中的作用研究[J].土壤学报,2026,63(1):230-240,11.基金项目
国家自然科学基金项目(42477057)和大学生创新创业训练计划项目(X202410635664)资助 Supported by the National Natural Science Foundation of China(No.42477057),and the College Students'Innovative Entrepreneurial Training Plan Program(No.X202410635664) （42477057）
