色谱2026，Vol.44Issue(3)：234-247,14.DOI:10.3724/SP.J.1123.2025.08011
人血清中30种磷脂酰胆碱与溶血磷脂酰胆碱同系物的液相色谱-串联质谱测定及其与冠心病的关联分析
Determination of 30 homologues of phosphatidylcholines and lysophosphatidylcholines in human serum by liquid chromatography-tandem mass spectrometry and their correlation analysis with coronary artery disease
摘要
Abstract
Phosphatidylcholine(PC)and lysophosphatidylcholine(LPC)homologues are closely associated with coronary atherosclerosis.Accurate determination of their contents can provide an important basis for the clinical diagnosis and prognosis of coronary artery disease(CAD).In this study,an analytical method based on liquid chromatography-tandem mass spectrometry was established,which enabled the simultaneous and accurate determination of 30 PC and LPC ho-mologues using only 10 μL of human serum.Methanol-acetonitrile-methyl tert-butyl methyl ether-water was used as the extraction system,and an XBridge C18 column was selected as the stationary phase.The mobile phase consisted of an acetonitrile-water mixture(1∶1,volume ratio)and iso-propanol,both containing 7.5 mmol/L ammonium formate and 0.15%(volume ratio)formic acid,and gradient elution was adopted for separation.Detection was performed using an electrospray ionization source in the positive ion mode with multiple reaction monitoring.Method validation results showed that the method exhibited a good linear relationship,with an average linear cor-relation coefficient of≥0.999 7 over a linear range of 0.125-100 μg/mL.The limits of detection and limits of quantification were 0.01-1.94 μg/mL and 0.03-6.48 μg/mL,respectively.The recoveries ranged from 85.4%to 114.3%,while the intra-day precision and inter-day precision were no more than 4.6%and 12.6%,respectively.Serum samples from 110 clinical volunteers who underwent coronary angiography were determined using this method.The average population concentration of PC homologues was 526.80 μg/mL,and that of LPC homologues was 73.67 μg/mL.Spearman correlation analysis revealed that PC and LPC homologues were closely correlated with the severity of CAD,as well as with related clinical biochemical and lipid metabolism indicators,suggesting that they could serve as potential CAD-related metabolites in clinical practice.Designed to meet clinical analysis needs,this method features small serum sample volume,simple operation,and excellent response.It can efficiently determine 30 PC and LPC homologues in human serum,providing an important reference for exploring the association between these two lipid classes and CAD,as well as the translational application of related biomarkers.关键词
磷脂酰胆碱/溶血磷脂酰胆碱/动脉粥样硬化/冠心病/液相色谱-串联质谱/脂质组学Key words
phosphatidylcholine/lysophosphatidylcholine/atherosclerosis/coronary artery dis-ease/liquid chromatography-tandem mass spectrometry(LC-MS/MS)/lipidomics分类
化学化工引用本文复制引用
李汶煜,刘朝阳,董军,杨睿悦,李红霞,陈文祥,王思明..人血清中30种磷脂酰胆碱与溶血磷脂酰胆碱同系物的液相色谱-串联质谱测定及其与冠心病的关联分析[J].色谱,2026,44(3):234-247,14.基金项目
中央高水平医院临床科研业务费(BJ-2022-125) （BJ-2022-125）
国家重点研发计划(2021YFE0114200) （2021YFE0114200）
国家自然科学基金(81501842) （81501842）
北京自然科学基金(7182145).National High Level Hospital Clinical Research Funding(No.BJ-2022-125) （7182145）
National Key R&D Program of China(No.2021YFE0114200) （No.2021YFE0114200）
National Natural Science Foundation of China(No.81501842) （No.81501842）
Beijing Natural Science Foundation(No.7182145). （No.7182145）
