卤虫与鲢仔鱼饲喂对开口期鳜消化功能的比较研究
COMPARATIVE STUDY ON THE DIGESTIVE FUNCTION OF MANDARIN FISH(SINIPERCA CHUATSI)FED WITH ARTEMIA AND SILVER CARP FRY AT THE INITIAL FEEDING STAGE
摘要
Abstract
The characteristic of mandarin fish(Siniperca chuatsi)on live prey during the initial feeding stage poses challenges to artificial breeding and increases production costs.To evaluate the feasibility of replacing live prey and to investigate its effects on digestive function,3 days post-hatch larvae were divided into two groups:a control group(HB)fed continuously with silver carp larvae,and an experimental group(AB)fed Artemia enriched with Schizochytrium from 3 to 6 days post-hatch,followed by silver carp larvae until 9 days post-hatch.Despite nutritional enrichment,the crude protein content of Artemia remained significantly lower than that of silver carp larvae.Growth performance was impaired in the AB group,with total length,specific growth rate(SGR),and survival rate(SR)all significantly reduced compared with the HB group.Except for pepsin,the activities of trypsin,lipase,and chitinase were significantly elevated in the AB group,indicating enhanced digestive stress.Histological examination revealed exfoliation and disorganization of the intestinal mucosa,shortened villi,and marked thickening of both intestinal walls and villi in the AB group.Transcriptomic analysis further demonstrated that differentially expressed genes in the AB group were enriched in immune-and apoptosis-related pathways,including IL-17,Toll-like receptor,TNF,NF-κB,and apoptosis signaling pathways.qPCR validation confirmed significant upregulation of key genes(il17ra1a,cxcl19,ccl20a.3,tnfb,casp7,casp10,and bax),aligning with transcriptomic trends.In conclusion,although nutrient-enriched Artemia may have short-term potential as an alternative initial diet,it can induce digestive stress and intestinal responses in mandarin fish larvae,thereby compromising growth and survival.This study provides useful references for optimizing feeding strategies at the initial stage and evaluating intestinal health in juvenile mandarin fish.关键词
开口期/卤虫/生长/消化功能/鳜仔鱼Key words
Initial feeding stage/Artemia/Growth/Digestive function/Larvae of mandarin fish分类
农业科技引用本文复制引用
冯婧昀,李晓洁,李云,郭慧,王珺,陶怡曦,苗晓敏,翟旭亮,薛洋,徐浩,腾树君..卤虫与鲢仔鱼饲喂对开口期鳜消化功能的比较研究[J].水生生物学报,2026,50(3):55-65,11.基金项目
重庆市水产科技创新联盟重点攻关项目(CQFTIU202601-1) （CQFTIU202601-1）
重庆市技术创新与应用发展专项重点项目(CSTB2025TIAD-KPX0082) （CSTB2025TIAD-KPX0082）
重庆市渔业资源增殖放流效果评价(YK-C202510063) （YK-C202510063）
渔业绿色循环发展项目:长江上游特色经济水产绿色循环关键技术研发(4142500014)资助[Supported by the Key Project of Chongqing Fishery Technology Innovation Union(CQFTIU202601-1) （4142500014）
Key Project of Technology Innovation and Application Development of Science and Technology Bureau of Chongqing Municipality(CSTB2025TIAD-KPX0082) （CSTB2025TIAD-KPX0082）
The Evaluation on the Effectiveness of Fishery Stock Enhancement Project of Chongqing Fisheries Technical Extension Center(YK-C202510063) （YK-C202510063）
Project on Green Circular Development of Fisheries:Key Technological Research on Green Circular Development of Characteristic Economic Aquatic Products in the Upper Reaches of the Yangtze River(4142500014)] （4142500014）
