水生生物学报2026，Vol.50Issue(3)：77-86,10.DOI:10.3724/1000-3207.2025.2025.0241
黄河三角洲潮间带螃蟹肠道菌群结构及功能的特征分析
CHARACTERISTICS OF STRUCTURE AND FUNCTIONS OF CRAB GUT MICROBIOTA IN INTERTIDAL ZONE FROM THE YELLOW RIVER DELTA
摘要
Abstract
As key benthic organisms in coastal intertidal ecosystems,crabs harbor gut microbiota play critical roles in host environmental adaptation.To explore how intertidal habitats heterogeneity influences the structure and function of crab gut microbiota in the Yellow River Delta,this study analyzed the diversity,compositions,interaction networks and potential functions of crab gut bacterial communities in low(LM),middle(MM),and high(HM)tidal marshes using 16S rRNA high-throughput sequencing.The results showed that:(1)The diversity of crab gut bacterial community varied among the three marshes,with the highest species diversity(Shannon index)in HM and the highest species rich-ness(Chao1 index)in LM.(2)The compositions and abundances of predominant bacteria were distinctly different across tidal marshes.LEfSe analysis showed that Proteobacteria(42.70％)and Actinobacteriota(12.21％)were the biomarker taxa in HM,while Firmicutes(57.64％),Bacteroidota(13.28％),and Desulfobacterota(4.23％)were the biomarker taxa in LM,respectively.(3)The topologies of the co-occurrence networks differed markedly.Specifically,the HM network exhibited lower connectivity and weaker clustering with reduced average degree(2.43),graph density(0.028),modularity(0.22),and fewer key nodes(10 OTUs)compared to those in MM and LM.(4)PICRUSt2 predic-tion indicated that bacterial community functions in HM differed from those in MM and LM,with significantly higher relative abundances of carbon and nitrogen metabolism,membrane transport,and signal transduction.In conclusion,this study revealed that intertidal habitat heterogeneity significantly affected the community structure,interaction network,and metabolic function of crab gut microbiota,providing a theoretical basis for analyzing the ecological adapta-bility mechanisms of macrobenthos in the Yellow River Delta wetland.关键词
潮间带/螃蟹/肠道菌群/生境异质性/黄河三角洲Key words
Intertidal zone/Crabs/Guts microbiota/Habitat heterogeneity/The Yellow River Delta分类
生物科学引用本文复制引用
王永琪,张佳琦,王志康,杨继松,王雪宏,管博,任中华,何洋..黄河三角洲潮间带螃蟹肠道菌群结构及功能的特征分析[J].水生生物学报,2026,50(3):77-86,10.基金项目
山东省自然科学基金面上项目(ZR2023MD088) （ZR2023MD088）
山东省高校青年创新团队项目(2022KJ118) （2022KJ118）
国家自然科学基金——山东省联合基金项目(42271055和U2106214)资助[Supported by the Fund for Natural Science Foundation of Shandong Province(ZR2023MD088) （42271055和U2106214）
Shandong Province Universities Youth Innovation Teams Project(2022KJ118) （2022KJ118）
National Natural Science Foundation of China(42271055 and U2106214)] （42271055 and U2106214）
