水生生物学报2026，Vol.50Issue(3)：87-99,13.DOI:10.3724/1000-3207.2025.2025.0359
克氏原螯虾幼虾对饲料维生素A需求量的研究
DIETARY VITAMIN A REQUIREMENT OF JUVENILE RED SWAMP CRAYFISH(PROCAMBARUS CLARKII)
摘要
Abstract
This study aimed to investigate the effects of dietary vitamin A and determine its requirement of juvenile red swamp crayfish(Procambarus clarkii).Six isonitrogenous,isolipidic,and isoenergetic experimental diets were formu-lated with analyzed vitamin A potencies of 11573(A1),20042(A2),26274(A3),37818(A4),55159(A5),and 79200(A6)IU/kg,respectively.These diets were fed to crayfish with an initial average body weight of(3.40±0.01)g for 8 weeks.The results revealed that weight gain rate and specific growth rate were significantly higher in groups A4 and A5 compared to the control group(A1)(P＜0.05),with the highest values observed in group A4.In contrast,feed conversion ratio and hepatosomatic index were significantly decreased(P＜0.05),reaching the lowest values in group A4.Compared to the control group,the total antioxidant capacity,catalase,and superoxide dismutase activities in the hepatopancreas were significantly enhanced(P＜0.05)in group A4.Serum aspartate aminotransferase and alanine aminotransferase activities decreased initially and then increased with rising vitamin A levels,both attaining their mini-mum in group A4.Simultaneously,the incidence of B-cells and R-cells in the hepatopancreas,intestinal villi length and width,and activities of hepatopancreatic trypsin,amylase,lipase,and intestinal trypsin were significantly elevated in group A4(P＜0.05).Furthermore,group A4 significantly up-regulated the gene expression levels of Chitinase,EcR,and RXR in the hepatopancreas,while significantly down-regulating the expression of MIH(P＜0.05).In conclusion,opti-mal vitamin A supplementation improve digestive function,enhance hepatopancreas antioxidant and immune capacity,and regulate ecdysis-related gene expression,thereby promoting growth performance in P.clarkii.Based on broken-line regression analysis of weight gain rate,specific growth rate,and feed conversion ratio,the recommended dietary vitamin A requirement for juvenile Procambarus clarkii is 37976-43443 IU/kg.关键词
维生素A/生长性能/非特异性免疫/克氏原螯虾Key words
Vitamin A/Growth performance/Non-specific immunity/Procambarus clarkii分类
农业科技引用本文复制引用
郑兴龙,马世豪,刘洋洋,宋长春,王晨曦,罗凯,郜卫华,田娟..克氏原螯虾幼虾对饲料维生素A需求量的研究[J].水生生物学报,2026,50(3):87-99,13.基金项目
国家重点研发计划(2023YFD2402000) （2023YFD2402000）
中央级公益性科研院所基本科研业务费专项资金(YFI202404) （YFI202404）
湖北省特色淡水产品产业链科技研发项目 （）
湖北技术创新计划(2024BBB099)资助[Supported by the National Key R & D Program of China(2023YFD2402000) （2024BBB099）
Central Public-interest Scientific Institution Basal Research Fund,YFI(YFI202404) （YFI202404）
Technology Research and Development Project of Characteristic Freshwater Products Industry Chain in Hubei Province （）
Hubei Technology Innovation Plan Project(2024BBB099)] （2024BBB099）
