水生生物学报2026，Vol.50Issue(3)：100-107,8.DOI:10.3724/1000-3207.2025.2025.0204
缺失ca12基因显著提高草鱼耐碳酸盐碱度胁迫能力
LOSS OF CA12 GENE SIGNIFICANTLY ENHANCES THE TOLERANCE OF GRASS CARP(CTENOPHARYNGODON IDELLA)TO CARBONATE ALKALINITY STRESS
摘要
Abstract
To explore the function of carbonic anhydrase 12(ca12)in grass carp(Ctenopharyngodon idella)response to carbonate alkalinity stress,we subjected grass carp to alkalinity stress at different NaHCO3 concentration gradients(0,20,40,60,80,100,150 mmol/L),and systematically analyzed the expression level of ca12 in various tissues(brain,heart,kidney,and gill)as well as the histopathological changes in gill and intestinal tissues.Compared with wild-type grass carp,chimeric grass carp edited by CRISPR/Cas9 technology had higher survival rate at different alkalinity levels and time points.Moreover,the expression levels of ca12 in the kidney and gill tissues were significantly downregu-lated with increasing alkalinity.Under stimulation with 60 mM/L NaHCO3,gill and intestinal tissues of chimeric grass carp showed adaptive morphology changes,with a significantly lower apoptosis rate than that of wild-type grass carp,and the survival rate of chimeric grass carp was also higher under this condition.The study indicates that high alkali-nity stress exerts adverse effects on grass carp,and the chimeric grass carp edited by CRISPR/Cas9 technology display significantly higher survival rates in high-alkalinity water than wild-type grass carp.This suggests that the ca12 plays an important regulatory role in grass carp under high-alkaline conditions,making it a critical target for grass carp adap-tion to high-alkaline environments.Loss of ca12 function significantly enhances the alkalinity tolerance of grass carp,providing a new strategy to improve the aquaculture rate in saline-alkali areas and expand the development space of the fishery industry.关键词
基因编辑/NaHCO3胁迫/组织病理学/草鱼Key words
Gene editing/NaHCO3 stress/Histopathology/Ctenopharyngodon idella分类
农业科技引用本文复制引用
张子瑜,刘慧敏,全睿,肖武汉,蔡孝廉..缺失ca12基因显著提高草鱼耐碳酸盐碱度胁迫能力[J].水生生物学报,2026,50(3):100-107,8.基金项目
农业生物育种国家科技重大专项(2023ZD0406505)资助[Supported by the Biological Breeding-National Science and Techno-logy Major Project(2023ZD0406505)] （2023ZD0406505）
