烟草科技2026，Vol.59Issue(3)：1-9,9.DOI:10.16135/j.issn1002-0861.2025.0428
基于非靶向代谢组学的霍氏肠杆菌固态发酵烟叶代谢物动态变化
Non-targeted metabolomics analysis on tobacco leaves during solid-state fermentation by Enterobacter hormaechei
摘要
Abstract
To elucidate the metabolic mechanism of Enterobacter hormaechei in improving the quality of fermented tobacco leaves,non-targeted metabolomics technology was employed to systematically analyze the transformation patterns and key functional pathways of metabolites during the solid-state fermentation procedure of tobacco leaves.The high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(HPLC-QTOF-MS)technology was applied and partial least squares discriminant analysis(PLS-DA)was conducted to screen differential metabolites in tobacco leaves(C3F-AB 2022)from Baoji tobacco-producing area of Shaanxi Province fermented by Enterobacter hormaechei for 0-72 h.The key metabolic pathways were identified using KEGG pathway analysis.The results showed that:1)A total of 945 metabolites were identified,151 of which were significantly differential metabolites,including glucose,amino acids,organic acids,and terpenoids,etc.2)The differential metabolites were enriched in 129 pathways,such as biosynthesis pathway of phenylalanine,tyrosine,and tryptophan and biosynthesis pathway of terpenoid and quinone.Among these,5 pathways including biosynthesis of terpenoids and quinones,metabolism of phenylalanine and metabolism of galactose were significantly related with the leaf quality improvement.Non-targeted metabolomics analysis revealed that the growth and metabolism of Enterobacter hormaechei drive the transformation of differential metabolites by enriching in key pathways including terpenoids synthesis and phenylalanine metabolism,thereby improve the quality of tobacco leaves.关键词
烟叶/霍氏肠杆菌/固态发酵/差异代谢物/非靶向代谢组学Key words
Tobacco/Enterobacter hormaechei/Solid-state fermentation/Differential metabolite/Non-targeted metabolomics分类
轻工纺织引用本文复制引用
王祯,张亚新,杨红静,宋凯,魏进彬,臧志鹏,高玉珍,刘晨,韩露,王梁..基于非靶向代谢组学的霍氏肠杆菌固态发酵烟叶代谢物动态变化[J].烟草科技,2026,59(3):1-9,9.基金项目
中国烟草总公司科技重点研发项目"加速烟叶醇化微生物资源发掘及其应用技术研究"(110202202006) （110202202006）
甘肃省重点研发计划项目"烟草源微生物靶向增香提质技术研究"(22YF11GA292) （22YF11GA292）
中国烟草实业发展中心科技项目"基于微生物的烟叶清香型风格定向重构技术开发"(ZYSYQ-2022-7). （ZYSYQ-2022-7）
