烟草科技2026，Vol.59Issue(3)：54-62,9.DOI:10.16135/j.issn1002-0861.2025.0478
球毛壳菌Y55的分离鉴定及其与烯酰吗啉复配对烟草黑胫病的防治效果
Isolation and identification of Chaetomium globosum Y55 and its control effect against tobacco black shank in combination with dimethomorph
摘要
Abstract
In order to screen biocontrol bacteria against tobacco black shank and enhance their control effects,antagonistic endophytic fungi against Phytophthora nicotianae were isolated from healthy tobacco tissues using the plate confrontation method and the one with the best control effect was identified.The antimicrobial spectrum and compatibility with dimethomorph of the strain were determined,and the control efficacy of its fermentation broth combined with dimethomorph against tobacco black shank was evaluated through indoor pot experiment.The results showed that a total of 37 antagonistic fungal strains against P.nicotianae were screened out.Among which strain Y55,identified as Chaetomium globosum based on morphological and molecular biological identification,exhibited the strongest inhibitory effect.The 10%C.globosum Y55 fermentation broth achieved an inhibition rate of 65.82%against P.nicotianae with a broad antimicrobial spectrum.The compatibility between C.globosum Y55 and dimethomorph was high and the inhibition rate of the combination of 10%C.globosum Y55 fermentation broth and 1.00 mg/L dimethomorph against P.nicotianae was 79.84%.In pot experiments,the treatment of the combination yielded a control efficacy of 70.95%against tobacco black shank,which was significantly higher than those of the single agent treatments.关键词
烟草疫霉/球毛壳菌/烯酰吗啉/生物防治Key words
Phytophthora nicotianae/Chaetomium globosum/Dimethomorph/Biological control分类
农业科技引用本文复制引用
王重阳,姚涛,任权,王睿,孙文秀,曹毅..球毛壳菌Y55的分离鉴定及其与烯酰吗啉复配对烟草黑胫病的防治效果[J].烟草科技,2026,59(3):54-62,9.基金项目
中国烟草总公司重大科技项目"烟草典型共栖微生物资源鉴定、特征解析与开发利用"[110202201004(JY-04)] （JY-04）
烟草行业烟草病虫害监测与综合治理重点实验室开放课题"印度梨形孢诱导烟草抗蚜虫的机制研究"(KLTPMIMT2022-20). （KLTPMIMT2022-20）
