新疆农业大学学报2025，Vol.48Issue(5)：371-383,13.DOI:10.20088/j.cnki.jxau.2025.05.003
基于网络药理学和转录组学探究沙棘多糖对炎症因子的调控作用
Exploring Regulation of Inflammatory Factors Sea Buckthorn Polysaccharides Based on Network Pharmacology and Transcriptomics
摘要
Abstract
Based on the preliminary research on the active components of sea buckthorn(Hippophae rhamnoi-des L.)conducted by our research group,as well as the active components of sea buckthorn obtained from the Traditional Chinese Medicine Systems Pharmacology(TCMSP)database,this study employed network pharma-cology to predict the targets and signaling pathways involved in the immunomodulatory effect of sea buckthorn.Protein-protein interaction(PPI)networks and"component-target-disease"networks were constructed,and soft-ware such as AutoDock was used for molecular docking to verify the binding affinity between key active compo-nents of sea buckthorn and core targets.Subsequent validation was performed using RAW264.7 macrophage cells.Results from network pharmacology analysis indicated that 28 chemical components were screened from sea buckthorn,which interacted with 53 common immunomodulatory targets.These components were mainly in-volved in signaling pathways including the tumor necrosis factor(TNF)signaling pathway and nuclear factor kappa-B(NF-κB)signaling pathway.Cellular experimental results confirmed that sea buckthorn could signifi-cantly enhance the immunomodulatory function of macrophages.Specifically,sea buckthorn treatment induced morphological changes in RAW264.7 cells,improved the proliferation and phagocytic capacity of macrophages,enhanced the secretion of nitric oxide(NO),and upregulated the expression levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)(P＜0.05).To further elucidate the primary mecha-nism underlying the enhanced immunomodulation by sea buckthorn components,transcriptomic analysis was per-formed.The results showed that compared with the blank control group,573 genes exhibited significant differen-tial expression in the sea buckthorn-treated group.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrich-ment analysis revealed that these differentially expressed genes were mainly enriched in cytokine-cytokine recep-tor interaction pathways and complement and coagulation cascades,and were involved in the regulation of signal-ing pathways such as interleukin-17(IL-17),TNF,and NF-κB.In conclusion,sea buckthorn exerts its immuno-modulatory effect primarily by regulating the NF-κB signaling pathway.关键词
沙棘活性成分/网络药理学/分子对接/转录组学/免疫调节Key words
active components of sea buckthorn/network pharmacology/molecular docking/transcrip-tomics/immunomodulation分类
农业科技引用本文复制引用
张小平,聂梅利,杨博轩,帕尔哈提·柔孜,阿衣吐逊·阿布都外力,艾合米丁·外力,蔡永国..基于网络药理学和转录组学探究沙棘多糖对炎症因子的调控作用[J].新疆农业大学学报,2025,48(5):371-383,13.基金项目
新疆维吾尔自治区自然科学基金青年基金项目(2022D01B89) （2022D01B89）
