眼科学报2026，Vol.41Issue(2)：105-113,9.DOI:10.12419/24110601
激活TREK-TRAAK K2P对氧化损伤人RPE细胞吞噬功能的保护作用
Protective effect of activating TREK-TRAAK K2P on the phagocytic function of oxidative damaged human RPE
摘要
Abstract
Objective:To observe the effect of TREK-TRAAK K2P activation on the phagocytic function of oxidative damaged human retinal pigment epithelial cells(RPE).Methods:Immunofluorescence staining was used to detect the expression of TREK-1,TREK-2 and TRAAK channel proteins in human RPE cells,and the RPE cell oxidative damage model was induced by tert-butyl hydroperoxide(t-BHP)at different time and concentration gradients.The study was divided into control group,t-BHP model group,riluzole plus t-BHP group,and riluzole group.2×107/mL fluorescent microspheres or FITC-labeled porcine photoreceptor outer segment membrane discs were added to each group for 6h incubation,fluorescence photos were obtained after fixation and staining,and the phagocytosis rate and phagocytosis index of RPE cells was analyzed and calculated by Image-Pro Plus 6.0 software.Results:TREK-1,TREK-2,and TREEK channel subtype proteins were all highly expressed in the human RPE cytoplasm.The survival rate of human RPE cells after t-BHP intervention was concentration-and time-dependent.There was no statistical difference in the survival rate between 200 μmol/L t-BHP intervention for 6 hours and the control group(P>0.05),and 400 μmol/L t-BHP intervention for 6 hours induced half death.Specific phagocytic index:The t-BHP group was lower than other groups(P<0.001).Specific phagocytosis rate:the t-BHP group was lower than other groups,with no statistical difference(P>0.05).Nonspecific phagocytic index:the t-BHP group was lower than other groups(P<0.001);nonspecific phagocytic rate:the t-BHP model group was lower than the riluzole plus t-BHP group,with no statistical difference(P>0.05),and only the riluzole group was higher than the t-BHP model group(P<0.05)in pairwise comparison.Conclusions:Activation of TREK-TRAAK K2P can protect the phagocytic function of human RPE cells damaged by oxidative stress.关键词
年龄相关性黄斑变性/氧化损伤/视网膜色素上皮细胞/吞噬功能/TREK-TRAAK K2PKey words
age related macular degeneration/oxidative stress/RPE/phagocytic function/TREK-TRAAK K2P分类
医药卫生引用本文复制引用
郑文斌,余清花,陈菁,李乃洋..激活TREK-TRAAK K2P对氧化损伤人RPE细胞吞噬功能的保护作用[J].眼科学报,2026,41(2):105-113,9.基金项目
广东省医学科学技术研究基金项目(2023102620446665) （2023102620446665）
中山市人民医院青年拔尖计划项目.This work was supported by the Guangdong Medical Science and Technology Research Fund Project(2023102620446665)and Zhongshan People's Hospital Youth Elite Program Project. （2023102620446665）
