新疆农业大学学报2025，Vol.48Issue(5)：426-435,10.DOI:10.20088/j.cnki.jxau.2025.05.009
不同养殖模式下牛源及环境源大肠杆菌耐药性与耐药基因分析
Antimicrobial Resistance and Resistance Gene Analysis of Escherichia coli from Cattle and Environmental Sources under Different Farming Systems
摘要
Abstract
To explore the impact of different cattle farming systems on antimicrobial resistance and associated resistance genes of Escherichia coli isolated from bovine and environmental sources under uniform management conditions.A total of 173 samples were collected,including rectal swabs from cattle and environmental samples from intensive and grazing systems.Escherichia coli isolates were obtained and tested for antimicrobial suscepti-bility to 10 antibiotics using the agar dilution method.PCR was employed to detect 21 commonly encountered an-timicrobial resistance genes.E.coli was isolated from 104 out of 173 samples,yielding an overall isolation rate of 60.1%.The highest rate was observed in bovine samples from intensive systems(82.0%),while the lowest occurred in environmental samples from intensive systems(36.5%).The isolates exhibited highest resistance rates to ampicillin(61.5%),florfenicol(66.3%),and ceftiofur(53.8%).Multidrug resistance(≥3 classes)was observed in 57.7%of isolates,with isolates from intensively raised cattle showing the most diverse resis-tance profiles.A total of nine antimicrobial resistance genes were detected,and the detection rates of blaTEM,floR,and sul2 in bovine-origin E.coli were significantly higher than those in environmental-origin isolates(P＜0.05).Under uniform management conditions,farming system significantly influenced both the antimicrobial re-sistance and gene carriage patterns of E.coli from bovine and environmental sources.Differential antimicrobial strategies should be tailored to specific farming contexts.关键词
大肠杆菌/不同养殖模式/耐药性/耐药基因Key words
Escherichia coli/different farming systems/drug resistance/resistance genes分类
农业科技引用本文复制引用
杨梦琪,韩俊成,夏盼盼,徐淑琴,王代冰,杨玉洁,田芮,夏利宁..不同养殖模式下牛源及环境源大肠杆菌耐药性与耐药基因分析[J].新疆农业大学学报,2025,48(5):426-435,10.基金项目
国家自然科学基金地区基金项目(32360910) （32360910）
新疆维吾尔自治区天山英才培养计划-"三农"骨干人才培养项目(2023SNGGGCC008) （2023SNGGGCC008）
