生态科学2026，Vol.45Issue(1)：19-29,11.DOI:10.14108/j.cnki.1008-8873.2026.01.003
低洼盐碱地稻蟹综合种养不同时期水稻根际土壤微生物群落动态变化
Dynamic variations of rice rhizosphere soil microbial community at different stages of integrated rice-crab coculture in low-lying saline-alkali land
摘要
Abstract
The study examines the dynamic variations in the diversity and structural composition of the rice rhizosphere soil microbial community,as well as the variations in soil physico-chemical properties,across different stages of integrated rice-crab coculture in low-lying saline-alkali land,which provides significant theoretical support for the amendment of saline-alkali land and the enhancement of crop quality.Illumina Miseq technology is utilized to sequence the V3-V4 region of bacterial 16S rRNA and the fungal ITS1-1F region in rhizosphere soil samples collected at the pre-planting,tillering,heading,and maturity stages of rice cultivation.The analysis indicates significant differences in the rhizosphere soil microbial communities among the other three growth stages compared to the pre-planting stage,as well as between the tillering and the heading and maturity stages,which tend to stabilize after the heading stage.The microbial community diversity and abundance are found to peak at the heading stage.A core group of rhizosphere microbial community is maintained across all stages,albeit with varying abundances.Proteobacteria,Bacteroidota,Gemmatimonadota,and Acidobacteriota are identified as the main dominant bacterial phyla,while Ascomycota,Rozellomycota,and Chytridiomycota are the main dominant fungal phyla.Characteristic microorganisms of the bacterial community at the pre-planting stage are Actinobacteriota,Chloroflexi,and Proteobacteria;at the heading stage,Nanoarchaeota and Verrucomicrobiota;at maturity,Bacteroidota,Desulfobacterota,and Myxococcota.In the fungal community,Mortierellomycota is the characteristic microorganism at the pre-planting stage,and Ascomycota,at the maturity stage.Soil pH and electrical conductivity decreases with the growth process of rice,and so does the total soil salinity level.The composition of the rhizosphere soil microbial community and multiple soil physico-chemical factors and soil enzyme activities exhibit dynamic variations in conjunction with the rice growth process under the integrated rice-crab coculture model in low-lying saline-alkali land.关键词
根际微生物/高通量测序/不同时期/低洼盐碱地/稻蟹综合种养/土壤理化性质Key words
rhizosphere microorganism/high-throughput sequencing/different stages/low-lying saline-alkali land/integrated rice-crab coculture/soil physico-chemical properties分类
农业科技引用本文复制引用
孙怡欣,刘祥婷,杨涓,石伟,王彬,田蕾,罗成科,邱小琮..低洼盐碱地稻蟹综合种养不同时期水稻根际土壤微生物群落动态变化[J].生态科学,2026,45(1):19-29,11.基金项目
国家重点研发计划专题(2021YFD1900603-03) （2021YFD1900603-03）
2023年大学生创新创业项目(S202310749083) （S202310749083）
