摘要
Abstract
Objective To investigate the potential molecular mechanisms of NPY1R in breast cancer.Methods The expression of NPY1R in breast cancer cell lines T47D,MCF-7,BT549,and MDA239 was detected by RT-qPCR and Western blot analysis.The expression of NPY1R in breast cancer MCF-7 cells was knocked down using lentiviral infection.Transcriptome sequencing of NPY1R-knockdown MCF-7 cells and control groups was performed using third-generation sequencing technology to screen for differentially expressed genes(DEGs).Subsequently,Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were conducted on the DEGs.Proteins were extracted from NPY1R-knockdown breast cancer cells and control groups,and differentially expressed proteins were identified.GO functional enrichment analysis and KEGG pathway enrichment analysis were also performed on the DEPs.Protein-protein interaction network analysis was conducted using the STRING database.Results Compared with the other three cell lines,NPY1R expression was significantly upregulated in MCF-7 cells.A total of 690 DEGs were identified.GO enrichment analysis revealed that upregulated DEGs were primarily associated with the muscle system,ossification,and heart contraction,while downregulated DEGs were mainly involved in positive regulation of cell adhesion,adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains,and leukocyte adhesion.KEGG enrichment analysis indicated that upregulated DEGs were predominantly enriched in neuroactive ligand-receptor interaction,pathways in cancer,and the cytoskeleton in muscle cells,whereas downregulated DEGs were mainly enriched in cell adhesion molecules,cytokine-cytokine receptor interaction,and neutrophil extracellular trap formation.In proteomic analysis,GO enrichment analysis showed that differentially expressed proteins were significantly enriched in RNA splicing,mRNA processing,and ribonucleoprotein complex biogenesis.KEGG enrichment analysis revealed that differentially expressed proteins were notably enriched in Shigellosis infection,Salmonella infection,and regulation of the actin cytoskeleton.PPI network analysis identified SRC as a key protein.Conclusion This study reveals the potential mechanisms of NPY1R in breast cancer and provides new insights for improving the prognosis of breast cancer.关键词
乳腺癌/NPY1R/生物信息学分析/SRCKey words
Breast cancer/NPY1R/Bioinformatics analysis/SRC分类
医药卫生
