中国动物检疫2026，Vol.43Issue(2)：8-14,7.DOI:10.3969/j.issn.1005-944X.2026.02.002
2024-2025年川西北牧区牦牛IBRV检测及分子特征分析
Detection and Molecular Characterization Aanalysis of Yak IBRV in Pastoral Areas of Northwestern Sichuan from 2024 to 2025
摘要
Abstract
In order to identify the prevalence and molecular characteristics of infectious bovine rhinotracheitis virus(IBRV)in yak herds in Northwestern Sichuan,50 nasal swab samples were collected from yaks with respiratory diseases on 12 pastures in 7 counties of the pastoral areas of the region from July 2024 to June 2025,to detect virus nucleic acids using a specific PCR assay.The results showed that IBRV was detected on 7 pastures in 4 counties,indicating the prevalence of IBRV in yak herds in the region.3 strains of viruses were isolated using MDBK cell culture,and confirmed as IBRV through PCR,indirect immunofluorescence assay and electron microscopy.Complete gene sequences of gC,gB and gD of the above strains were obtained using viral metagenomic sequencing,indicating that all the strains were serotype 1.2;further analysis revealed that the genotype of the isolates was different from that of domestic vaccine strain(subtype 1.1),but no significant variations were observed in the key antigenic regions of the major immunogenic proteins gB and gD.A reference was provided for future prevention and control of IBRV infection in yaks in the pastoral areas of Northwestern Sichuan.关键词
传染性牛鼻气管炎病毒/牦牛/川西北/病毒分离/分子特征Key words
IBRV/yak/Northwestern Sichuan/virus isolation/molecular characteristic分类
农业科技引用本文复制引用
陈玉莹,张朝辉,徐林,余劲,汤承,岳华..2024-2025年川西北牧区牦牛IBRV检测及分子特征分析[J].中国动物检疫,2026,43(2):8-14,7.基金项目
"十四五"国家重点研发计划项目(2023YFD180250401) （2023YFD180250401）
