中国动物检疫2026，Vol.43Issue(2)：29-33,61,6.DOI:10.3969/j.issn.1005-944X.2026.02.005
羊口疮病毒四川流行株B2L基因及抗原表位分析
Analysis of the B2L Gene and Antigenic Epitopes of Sichuan Strains of Orf Virus
摘要
Abstract
In order to investigate the molecular characteristics of the B2L gene of Orf virus(ORFV)prevalent in Sichuan province,the sequences and antigenic epitopes of ORFV B2L gene were analyzed based on 8 positive samples collected from goat farms in Liangshan prefecture,Meishan city and Jianyang city.The results showed that 6 sequences of B2L gene clustered into 2 distinct branches on the phylogenetic tree;8 linear B-cell epitopes,9 cytotoxic T-cell(CTL)epitopes and 4 helper T-cell(HTL)epitopes were contained in the sequences of B2L protein.Compared with domestic ORFV reference strains,the conservation of 5 linear B-cell epitopes,7 cytotoxic T-cell epitopes and 3 helper T-cell epitopes was greater than 90％.In conclusion,the ORFV B2L gene of the strains prevalent in 3 regions in the province presented genetic diversity,and its key antigenic epitopes were highly conserved.By the study,the molecular characteristics of ORFV strains prevalent in Sichuan province were enriched,and a reference was provided for future prevention and control of ORFV infection as well as development of novel vaccines.关键词
羊口疮病毒/B2L基因/抗原表位/保守性/四川省Key words
ORFV/B2L gene/antigenic epitope/conservation/Sichuan province分类
农业科技引用本文复制引用
沈少鹏,曹琪慧,岳华,汤承..羊口疮病毒四川流行株B2L基因及抗原表位分析[J].中国动物检疫,2026,43(2):29-33,61,6.基金项目
"十四五"国家重点研发计划项目(2021YFD 1600203) （2021YFD 1600203）
西南民族大学基本科研业务费专项(ZYN2024180) （ZYN2024180）
