畜牧与兽医2026，Vol.58Issue(4)：8-15,8.
宣和猪ZFAT基因外显子7多态性及其对胴体和肉质性状的影响
Polymorphisms of the exon 7 of ZFAT gene and its effects on carcass and meat traits in Xuanhe pigs
摘要
Abstract
To identify the polymorphisms of exon 7 in the ZFAT gene and its effect on meat traits in Xuanhe pigs,this study used first se-quencing technology to detect single nucleotide polymorphisms(SNPs)in exon 7 of the ZFAT gene in 106 Xuanhe pigs.Different genotypes and haplotype combinations were then associated with carcass and meat quality traits using a least squares model.The results showed that one missense mutation site(G3174A)and four synonymous mutation sites(C2693T,C2834T,T3173C,and C3224T)were identified in the ex-on 7 region of the ZFAT gene,and all were in a Hardy-Weinberg equilibrium state(P＞0.05).Except the C2693T and G3174A sites,the C2834T,T3173C and C3224T sites exhibited moderate polymorphism(0.3076≤PIC≤0.3739)and rich genetic diversity(0.3797≤H≤0.497 8)in Xuanhe pigs.The results of the association analysis showed that the C2693T,C2834T and T3173C sites were significantly asso-ciated with average backfat thickness and ham percentage(P＜0.05 or P＜0.01)in Xuanhe pigs.The C2693T,C2834T,T3173C and G3174A sites were significantly associated with meat color score,pH value,drip loss,and intramuscular fat content(P＜0.05 or P＜0.01)in Xuanhe pigs.Different haplotype combinations were significantly associated with carcass traits(carcass straight length,carcass diagonal length,ham percentage,lean percentage,dressing percentage),and meat traits(meat color score,marbling score,pH value,cooked rate,and drip loss)(P＜0.05 or P＜0.01)in the pigs.Meanwhile,the individuals carrying CCCGT and CCTGC haplotypes exhibited high carcass and meat quality.These results indicated that polymorphisms of exon 7 in ZFAT gene were significantly associated with carcass and meat traits in Xuanhe pigs,which would serve as a theoretical basis for using this gene marker to improve the meat quality traits in pigs.关键词
宣和猪/ZFAT基因/单核苷酸多态性/胴体性状/肉质性状Key words
Xuanhe pig/ZFAT gene/single nucleotide polymorphism/carcass trait/meat trait分类
农业科技引用本文复制引用
陈阳,鲁绍雄,朱怡轩,王如玉,吴娅,聂子棋,李明丽,张克忠,王孝义,陈强..宣和猪ZFAT基因外显子7多态性及其对胴体和肉质性状的影响[J].畜牧与兽医,2026,58(4):8-15,8.基金项目
云南省生猪产业技术体系项目(2024KJX0016) （2024KJX0016）
云南省宣威市宣和猪产业科技特派团项目(202104BI090022) （202104BI090022）
国家重点研发计划项目(2022YFD1601902) （2022YFD1601902）
