中国临床药理学杂志2026，Vol.42Issue(1)：40-46,7.DOI:10.13699/j.cnki.1001-6821.2026.01.007
柯里拉京通过转录因子7影响足细胞氧化应激及凋亡缓解糖尿病肾病的研究
Research of corilagin alleviating diabetic nephropathy by influencing podocyte oxidative stress and apoptosis by transcription factor 7
摘要
Abstract
Objective To explore the possible mechanisms through which corilagin affects high-glucose-induced oxidative stress and apoptosis in podocytes to alleviate diabetic nephropathy(DN).Methods MPC5 cells in the logarithmic growth phase were divided into 5 groups:control group(5 mmol·L-1 glucose),model group(30 mmol·L-1 glucose),corilagin group(30 mmol·L-1 glucose plus 50 μM corilagin treatment),corilagin+pcDNA3.1 group(30 mmol·L-1 glucose plus corilagin 50 μM treatment after transfection with pcDNA3.1),and corilagin+pcDNA3.1-transcription factor 7(TCF7)group(30 mmol·L-1 glucose plus corilagin 50 μM treatment after transfection with pcDNA3.1-TCF7).Real-time quantitative polymerase chain reaction(qPCR)was used to detect the expression of long non-coding RNA(LncRNA)TCF7;terminal deoxynucleotidyl transferase dUTP nick-end labeling was employed to assess the apoptosis rate;Western blot was used to analyze the expression of Cleaved caspase-9,Cleaved caspase-3,and proteins related to the glycogen synthase kinase 3 beta(GSK3β)/nuclear factor erythroid-2-related factor2(Nrf2)signaling pathway;2′,7′-dichlorofluorescein diacetate fluorescent probe was utilized to measure reactive oxygen species(ROS)levels;and immunofluorescence was used to examine the expression of nephrin and podocin.Results The apoptosis rates for the control,model,corilagin,corilagin+pcDNA3.1 and corilagin+pcDNA3.1-TCF7 groups were(5.72±0.49)％,(31.14±5.46)％,(17.01±2.75)％,(16.21±2.83)％and(25.80±3.42)％,respectively;the relative expression levels of Cleaved caspase-9 were 1.00±0.11,3.91±0.41,2.43±0.29,2.49±0.33 and 3.88±0.37,respectively;the relative expression levels for Cleaved caspase-3 were 1.00±0.08,5.04±0.63,3.35±0.48,3.29±0.45 and 4.78±0.56,respectively;the ROS levels were 1.00±0.14,6.27±1.16,2.29±0.32,2.41±0.38 and 5.17±1.08,respectively;malondialdehyde(MDA)levels were(2.33±0.35),(9.26±2.23),(4.80±0.86),(4.47±0.74)and(7.52±1.36)nmol·mg-1,respectively;superoxide dismutase(SOD)levels were(41.34±6.13),(12.15±1.99),(33.38±4.55),(30.01±4.70)and(24.16±3.71)U·mg-1,respectively;and catalase(CAT)levels were(68.22±5.45),(28.27±3.48),(52.13±6.06),(50.24±7.43)and(41.91±5.39)U·mg-1,respectively.When the above indicators in control group compared with those in model group,the above indicators in model group compared with those in the corilagin group,and the above indicators in the corilagin+pcDNA3.1 group compared with those in the corilagin+pcDNA3.1-TCF7 group,all differences showed statistically significant(P＜0.05,P＜0.01,P＜0.001).Conclusion Corilagin can inhibit high-glucose-induced oxidative stress and apoptosis in podocytes,thereby alleviating DN,possibly related to its regulation of the GSK3β/Nrf2 signaling pathway by LncRNA TCF7.关键词
柯里拉京/糖尿病肾病/转录因子7/糖原合成酶激酶3β/核因子E2相关因子2/足细胞/氧化应激/凋亡Key words
corilagin/diabetic nephropathy/transcription factor 7/glycogen synthase kinase 3 beta/nuclear factor erythroid-2-related factor2/podocyte/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
桑乾才让,扎西措,慕晓旭,才让吉,李先加..柯里拉京通过转录因子7影响足细胞氧化应激及凋亡缓解糖尿病肾病的研究[J].中国临床药理学杂志,2026,42(1):40-46,7.基金项目
第七批全国老中医药专家学术经验继承工作基金资助项目(2022-2408) （2022-2408）
2023年青海'昆仑英才'中青年科技人才托举工程基金资助项目(2023QHSKXRCTJ42) （2023QHSKXRCTJ42）
青海省藏医院院级科研基金资助项目(ZYKY-2024-10) （ZYKY-2024-10）
