中国实验动物学报2026，Vol.34Issue(2)：168-184,17.DOI:10.3969/j.issn.1005-4847.2026.02.002
小型猪脑卒中模型构建的可行性研究
Feasibility study on the construction of a small porcine stroke model
摘要
Abstract
Objective This study aimed to establish minipig models of ischemic stroke(IS)and hemorrhagic stroke(HS)with high clinical relevance,to evaluate the feasibility of using minipigs as in vivo stroke models by monitoring post-stroke pathophysiological processes,and to provide a more accurate experimental platform for investigating stroke mechanisms and developing therapeutic strategies.Methods The HS model was established by autologous blood injection using a double-injection method,while the IS model was constructed via cranial vascular electrocoagulation.After surgery,behavioral assessments,physiological and biochemical indices,histopathological examination of brain tissue,and immunohistochemical analysis of neuronal markers were conducted to comprehensively evaluate the extent to which the models replicated clinical stroke progression.Results Pigs in the IS model exhibited severe hemiparesis,motor dysfunction,and reduced levels of consciousness,accompanied by marked ischemic necrosis of brain tissue,pronounced inflammatory responses,significant neuronal apoptosis,and mild impairment of liver and kidney function.By contrast,pigs in the HS model showed mild behavioral disturbances and stable hematoma formation,along with cerebral edema,mechanical compression,active inflammatory responses,and evident neuronal injury with glial cell activation.Both models demonstrated good stability and reproducibility.Conclusions IS and HS minipig models with high clinical relevance were successfully established in this study.These findings confirm the feasibility and reliability of constructing stroke models in minipigs and provide a robust experimental basis for the development of targeted therapeutic strategies,supporting translational research and the advancement of precision stroke therapy.关键词
小型猪模型/两次注血法/开颅血管电凝/缺血性脑卒中/出血性脑卒中Key words
minipig model/double-pass blood transfusion technique/cranial vascular electrocoagulation/ischemic stroke/hemorrhagic stroke分类
生物科学引用本文复制引用
郭鑫旺,李开通,高宏志,魏红江,赵红业,王佳音,高如玉,代淑玲,陈晓晶,吴保方,程文杰,魏太云,赵耀博..小型猪脑卒中模型构建的可行性研究[J].中国实验动物学报,2026,34(2):168-184,17.基金项目
云南省重大科技专项计划(202102AA100054).Funded by Yunnan Province Major Science and Technology Special Programme(202102AA100054). （202102AA100054）
