中国实验动物学报2026，Vol.34Issue(2)：195-205,11.DOI:10.3969/j.issn.1005-4847.2026.02.004
杜仲皮与杜仲叶对抑郁症大鼠炎症反应及海马ZO-1/Occludin的作用比较
Comparative effects of Eucommia ulmoides bark and leaf on inflammatory response and hippocampal ZO-1/Occludin in depressive rats
摘要
Abstract
Objective Exploring the effects and characteristics of Eucommia ulmoides(E.ulmoides)bark and leaveson inflammation and blood-brain barrier damage in rats with depression.Methods the chronic unpredictable mild stress(CUMS)was used to establish the rat model of depression,the SD rats were randomly divided into control group,depressed group,E.ulmoides bark group and E.ulmoides leaf group.Behavioral tests were carried out by using open field and Morris water maze experiments;hematoxylin-eosin(HE)and Nissl staining were used to observe the pathological morphology of the hippocampus(HP)of the rats;ELISA was used to detect the contents of IL-1β,IL-6,and TNF-α in the serum and hippocampus of the rats;The expression levels of ZO-1 and Occludin were detected by immunofluorescence.Results Compared with the control group,the target quadrant residence time,crossing platforms,the number of climbing frames and standing frequency of rats in the depressed group were significantly lower(P＜0.05 or P＜0.01);There are obvious cellular structural blurriness,inflammatory cell infiltration and damage,decreased or even disappeared Nissl bodies,and degeneration and necrosis of nerve cells in hippocampal tissue;the contents of IL-1β,IL-6,and TNF-α were significantly elevated(P＜0.01);the positive expression of ZO-1 and Occludin was reduced,and the fluorescence intensity was reduced(P＜0.05).Compared with the depressed group,the residence time in the target quadrant,the number of crossing platforms,and the number of climbing frames of rats in the E.ulmoides bark group were significantly higher(P＜0.05 or P＜0.01),the number of crossing platforms,the number of climbing frames and standing frequency ofrats in the E.ulmoides leaf group were significantly higher(P＜0.05 or P＜0.01);the pathological morphology was restored;and the contents of IL-1β,IL-6,and TNF-α in serum and hippocampus of the rats in the E.ulmoides bark and E.ulmoides leaf group were significantly lower(P＜0.01 or P＜0.05);positive expression of ZO-1 and Occludin was elevated(P＜0.05).Conclusions Both E.ulmoides bark and E.ulmoides leaves can significantly improve the behavior of depressed rats,regulate blood-brain barrier permeability,reduce inflammatory response damage,and thus protect neurological function.关键词
抑郁症/血脑屏障/炎症反应/杜仲Key words
depression/blood-brain barrier/inflammatory response/Eucommia ulmoides分类
生物科学引用本文复制引用
陈雨立,王鑫,刘羽,赵洪庆,易亚乔,周伟良,王汝琴,刘林..杜仲皮与杜仲叶对抑郁症大鼠炎症反应及海马ZO-1/Occludin的作用比较[J].中国实验动物学报,2026,34(2):195-205,11.基金项目
湖南省重点研发项目(2023DK2005,2024JK2121),湖南省自然科学基金(2025JJ80956,2023JJ60488),湖南省科技特派员项目(2024RC8215),产学研横向课题(2024430119000475,2024430119000477,2024430119000478,2024430119000474),湖南省教育厅项目(22A0262,24B0344).Funded by Key Research and Development Project of Hunan Province(2023DK2005,2024JK2121),Natural Science Foundation of Hunan Province(2025JJ80956,2023JJ60488),Science and Technology Commissioner Project of Hunan Province(2024RC8215),Industry-University-Research Horizontal Projects(2024430119000475,2024430119000477,2024430119000478,2024430119000474),Education Department of Hunan Province(22A0262,24B0344). （2023DK2005,2024JK2121）
