中国实验动物学报2026，Vol.34Issue(2)：206-212,7.DOI:10.3969/j.issn.1005-4847.2026.02.005
无菌中国地鼠模型建立
Establishment of germ-free Chinese hamster model
摘要
Abstract
Objective To establish a germ-free Chinese hamster model using cesarean section-based purification techniques.Methods Female SPF Chinese hamsters were mated with males at a 1∶1 ratio,after which the males were separated following copulation.Surrogate mother rats were introduced into the experimental environment 1～2 d prior to the donor females.At term,pregnant Chinese hamsters underwent hysterectomy under a super-clean workbench and were immediately transferred to isolation units for purification.The uteri were dissected to obtain neonatal pups,which were subsequently fostered by germ-free(GF)grade ICR mice.After successful fostering,the pups were transferred to isolation units for continued rearing.Sterility of the isolation units was monitored monthly.Results Two cesarean sections were performed.The first fostering attempt was unsuccessful,whereas the second was successful,yielding nine pups with a survival rate of 56％.All GF-grade Chinese hamsters met the requirements of GB/T 14926.41-2001.Conclusions Using GF-grade ICR mice as foster mothers in combination with cesarean section purification technology,microbial decontamination was successfully achieved,resultsing in the establishment of a of Chinese hamster model.关键词
中国地鼠/无菌动物/剖宫产净化/隔离器Key words
Chinese hamster/germ-free animals/cesarean section purification/isolator分类
生物科学引用本文复制引用
郭亚茜,刘凯慧,杜晓鹏,王昭华,朱华..无菌中国地鼠模型建立[J].中国实验动物学报,2026,34(2):206-212,7.基金项目
中国医学科学院医学与健康科技创新工程项目(2022-12M-1-020).Funded by CAMS Innovation Fund for Medical Sciences(CIFMS)(2022-12M-1-020). （2022-12M-1-020）
