中国实验动物学报2026，Vol.34Issue(2)：213-219,7.DOI:10.3969/j.issn.1005-4847.2026.02.006
建立稳定的肺动脉高压小鼠模型的新方法
A novel approach for developing a stable pulmonary arterial hypertension mouse model
摘要
Abstract
Objective To develop a reliable mouse model of pulmonary hypertension(PH)using monocrotaline(MCT).Methods Female C57BL/6 mice aged 6～8 weeks were selected and administered MCT through intraperitoneal injection once weekly for three weeks.On days 10,20,and 30 after the final injection,the mice were evaluated.A 0.55 mm x 20 mm disposable infusion needle was inserted through the intercostal muscles to access the right ventricle,and the right ventricular systolic pressure(RVSP)was recorded with the medlab biological signal acquisition system.The heart was dissected,the body mass(BM),right ventricle(RV),and left ventricle plus septum(LV+S)were measured,and then the right ventricular hypertrophy index(RVHI)=RV/BM and fulton index(RV/LV+S)were calculated.Lung tissue sections were prepared for pathological analysis.Then,immunofluorescence(IF)staining was performed for hematoxylin-eosin(HE)and α-smooth muscle actin(α-SMA)to examine changes in pulmonary vascular structure.Results At each of the three time points,RVSP,RVHI,and RV/LV+S values in MCT group mice were markedly higher than those in the control group.HE staining confirmed significant thickening of the pulmonary vascular walls,while IF staining revealed the expression levels of α-SMA proteins were significantly increased whereas the expression levels of CD31 proteins were significantly decreased.Conclusions Administering a consistent dose of MCT via weekly intraperitoneal injections for three weeks can create a stable mouse model of pulmonary hypertension over 30 d.关键词
肺动脉高压/野百合碱/小鼠模型/腹腔注射/肋间肌穿刺/α-SMAKey words
pulmonary hypertension/monocrotaline/mouse model/intraperitoneal injection/intercostal muscle puncture/α-SMA分类
生物科学引用本文复制引用
郭思佳..建立稳定的肺动脉高压小鼠模型的新方法[J].中国实验动物学报,2026,34(2):213-219,7.基金项目
福建省自然科学基金项目(2024J01672),福建省卫生领域科技创新联合项目(2024Y9366).Funded by Fujian Provincial Natural Science Foundation(2024J01672),Scientific and Technological Innovation Joint Capital Projects in the Health Field of Fujian Province(2024Y9366). （2024J01672）
