中国比较医学杂志2026，Vol.36Issue(4)：1-11,11.DOI:10.3969/j.issn.1671-7856.2026.04.001
基于P2X7R探讨冰水泳浴条件下高尿酸血症致痛风性关节炎发作的机制研究
Mechanism of gouty arthritis induced by hyperuricemia under ice-water swimming conditions based on P2X7R
摘要
Abstract
Objective To explore the mechanism of gout attacks in hyperuricemic rats under ice-water swimming conditions based on the purinergic P2X7 receptor(P2X7R).Methods Male Sprague-Dawley rats were divided randomly into Control group(n=6),HPD+IWS group(n=12),HPD group(n=6),and IWS group(n=6).Hyperuricemia was induced by gavage with potassium oxonate combined with yeast extract.Ice-water swimming was performed by 5-minute endurance swimming in an ice-water mixture.The experiment lasted for 21 days.Serum uric acid levels were detected by the uricase method,serum and liver xanthine oxidase(XOD)levels were detected by colorimetry,and serum ATP levels were detected by phosphomolybdic acid colorimetry.Uric acid crystals in joint fluid were observed by polarized light microscopy.During the experiment,the right ankle joint diameter and foot thickness were measured every 2 days,and the swelling index was calculated.The pathological changes of ankle joint and synovial tissue were observed by hematoxylin-eosin(HE)staining.The serum levels of interleukin(IL)-1β,IL-6,and tumor necrosis factor(TNF)-α were detected by ELISA.Neutrophil count in venous blood was detected by full self-help blood cell analyzer.The expression level of P2X7R protein in synovial tissue was detected by Western blot.The expression level of nucleotide-binding oligomerization domain-like receptor 3(NLRP3)protein in synovial tissue was detected by immunohistochemistry.Results Compared with Control group,on the 7th,14 th and 21 st days of the experiment,the serum uric acid levels of HPD+IWS group and HPD group were significantly increased(P＜0.01 or P＜0.05).Compared with HPD+IWS group,the serum uric acid level of HPD group was significantly decreased on the 7th and 21st days of the experiment(P＜0.01).And the serum uric acid level of IWS group was significantly decreased on the 7th,14th and 21st days of the experiment(P＜0.01).Compared with Control group,the serum and liver XOD levels in HPD+IWS group were significantly increased(P＜0.01).Compared with HPD+IWS group,the serum XOD levels of HPD group and IWS group were significantly reduced(P＜0.01).And the liver XOD level of HPD group was significantly reduced(P＜0.05).Compared with HPD+IWS group,the serum ATP of HPD group was significantly increased on the 7th,14th and 21st days(P＜0.01 or P＜0.05).And the serum ATP level of IWS group was significantly increased on the 14th day(P＜0.01).Urate crystals were observed in HPD+IWS group.Compared with Control group,the swelling index of the right ankle joint in HPD+IWS group increased significantly on the 5th,7th,9th,11th and 13th days(P＜0.01 or P＜0.05).Compared with Control group,the foot thickness swelling index of HPD+IWS group was significantly increased on the 5th,7th,9th,11th,13th,15th,17th,19th and 21st days(P＜0.01 or P＜0.05).The result of HE staining showed that synovial hyperplasia and inflammatory cell infiltration appeared in the ankle joint cavity of HPD+IWS group.Compared with Control group,the levels of serum IL-1β,IL-6 and TNF-α in HPD+IWS group were significantly increased(P＜0.01).Compared with HPD+IWS group,the levels of serum IL-1β,IL-6 and TNF-α in HPD group and IWS group were significantly decreased(P＜0.01).Compared with Control group,the neutrophil count in HPD+IWS group was significantly increased(P＜0.01).Compared with HPD+IWS group,the neutrophil count in IWS group was significantly reduced(P＜0.01).Compared with Control group,the expression of P2X7R and NLRP3 protein in the synovial tissue of HPD+IWS group was significantly increased(P＜0.01 or P＜0.05).Conclusions Ice-water swimming under hyperuricemic conditions can lead to ATP metabolic imbalance,increase uric acid levels and urate deposition,and activate P2X7R,leading to gouty arthritis attacks via the ATP-P2X7R-NLRP3 pathway.关键词
痛风性关节炎/P2X7R/尿酸/冰水泳浴/炎症因子Key words
gouty arthritis/P2X7R/uric acid/ice-water swimming/inflammatory cytokines分类
医药卫生引用本文复制引用
陈思颖,王寅峰,鲁程锦,蔡小晓,张冰,林志健..基于P2X7R探讨冰水泳浴条件下高尿酸血症致痛风性关节炎发作的机制研究[J].中国比较医学杂志,2026,36(4):1-11,11.基金项目
国家中医药管理局高水平重点学科建设项目-临床中药学(zyyzdxk-2023257) （zyyzdxk-2023257）
北京中医药薪火传承"新3+3"工程(2023-ZYSF-13) （2023-ZYSF-13）
北京中医药大学研究生自主科研课题(ZJKT2025033). （ZJKT2025033）
