中国兽医杂志2026，Vol.62Issue(3)：85-94,10.DOI:10.20157/j.cnki.zgsyzz.2026.03.010
核酸适配体在病毒性动物疾病检测中的研究进展
Research Progress on Nucleic Acid Aptamers in the Detection of Viral Animal Diseases
摘要
Abstract
Effective prevention and control of animal diseases are crucial for the sustainable development of the livestock industry and global public health security.In recent years,nucleic acid aptamer technology has attracted widespread attention in the field of animal disease diagnostics due to its high affinity and specificity for target molecules.Compared with traditional antibodies,nucleic acid aptamers offer advantages such as high chemical stability,low production cost,strong designability,and low immunogenicity,which have promoted their extensive application in animal disease detection.This paper provides a detailed overview of the definition,classification,and characteristics of nucleic acid aptamers,along with the selection process of systematic evolution of ligands by exponential enrichment(SELEX)technology.Furthermore,the current applications of nucleic acid aptamers in the detection of viral animal diseases are summarized.The challenges and future development directions of aptamer-based technologies are also discussed,aiming to provide a reference for research in animal disease prevention and control.关键词
核酸适配体/动物疾病/诊断/指数富集配体系统进化(SELEX)/公共卫生Key words
nucleic acid aptamer/animal disease/diagnosis/systematic evolution of ligands by exponential enrichment(SELEX)/public health分类
农业科技引用本文复制引用
李志豪,余新刚,黄福强,高寒,张克山,张苗苗,赵登率,李平,张远航,詹小舒,陈胜锋,覃丽梅,赵孟孟..核酸适配体在病毒性动物疾病检测中的研究进展[J].中国兽医杂志,2026,62(3):85-94,10.基金项目
国家重点研发计划(2023YFD1801300) （2023YFD1801300）
广东省基础与应用基础研究基金区域联合基金项目(2024A1515140152) （2024A1515140152）
广东省农业农村厅乡村振兴战略专项资金(2024CXTD15) （2024CXTD15）
广东省分子设计与精准育种重点实验室开放课题(2024B03) （2024B03）
佛山高层次人才启动项目(BKS111000,CGZ07001) （BKS111000,CGZ07001）
