种子2026，Vol.45Issue(2)：193-199,204,8.DOI:10.16590/j.cnki.1001-4705.2026.02.193
斑皮柠檬桉组培快繁技术体系构建研究
Research on the Tissue Culture and Rapid Propagation Technology System of Corymbia citriodora subsp.variegata
摘要
Abstract
In order to establish an efficient in vitro rapid propagation system for the Corymbia citriodo-ra subsp.variegata,stem segments of sterile seedlings of this species were used as explants.The key steps such as non-stem bud induction,proliferation,rooting medium,and acclimatization and trans-plantation were systematically studied.The results showed that the disinfection of Corymbia citriodo-ra subsp.variegata seeds using a combination of 75％alcohol and 0.1％mercuric chloride resulted in high seed survival rate and low contamination rate.The optimal medium for non-stem bud induction was:2 DCR+1.00 mg/L 6-BA+0.01 mg/L NAA,with an induction rate of 100％,without brown-ing or glassification phenomena.The optimal medium for subculture proliferation was:2 DCR+0.30 mg/L 6-BA+0.05 mg/L NAA,with a proliferation coefficient of 15.14,and the seedlings grew robustly without the need for vigorous seedling cultivation.The optimal rooting medium was:1/2 DCR+1.00 mg/L IBA+0.50 mg/L NAA+0.50 mg/L ABT,with a rooting rate of 93.33％and an average root length of 2.96 cm.The most suitable substrate ratio for transplantation was:Vhumussoil∶Vred soil∶Vpeat soil=1∶1∶1),with an survival rate of 95.24％.关键词
斑皮柠檬桉/无菌实生瓶苗/诱导率/组培快繁Key words
Corymbia citriodora subsp.variegata/sterile bottle seedling/induction rate/tissue culture rapid propagation分类
农业科技引用本文复制引用
王鸿嶙,吕少念,侯玉帆,周玉吉,孔令荣,陆庄跃,何真敏,曹子林,王晓丽..斑皮柠檬桉组培快繁技术体系构建研究[J].种子,2026,45(2):193-199,204,8.基金项目
云南省高校服务重点产业科技专项项目(FWCY-ZNT2024022) （FWCY-ZNT2024022）
云南省种子种业联合实验室项目(202205AR070001-22) （202205AR070001-22）
西南林业大学科研启动基金(112005) （112005）
