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烟草PILS基因家族鉴定及表达模式分析

刘翀 梁书苑 王飞雁 刘洋 鲍亚宁

种子2026,Vol.45Issue(1):1-11,11.
种子2026,Vol.45Issue(1):1-11,11.DOI:10.16590/j.cnki.1001-4705.2026.01.001

烟草PILS基因家族鉴定及表达模式分析

Identification and Expression Pattern Analysis of PILS Gene Family in Nicotiana tobacum

刘翀 1梁书苑 1王飞雁 1刘洋 1鲍亚宁1

作者信息

  • 1. 贵州大学烟草学院,贵州省烟草提质增效全省重点实验室,贵阳 550025
  • 折叠

摘要

Abstract

PIN-LIKE(PILS)proteins are a type of auxin transport proteins,of which specific localiza-tion on the cell membrane determines the direction of auxin flow between cells and the distribution of auxin content.To explore the functions of the PILS gene family of Nicotiana tabacum,using common Nicotiana tabacum as the research object,the NtPILS gene family was systematically identified at the whole-genome level,and its evolutionary relationship,physicochemical properties,chromosomal lo-calization,promoter cis-acting elements,tissue expression patterns,and response patterns under non-biological stress were analyzed using bioinformatics methods.The results showed that a total of 16 NtPILS genes were identified,among which 15 were distributed on 9 chromosomes(3,4,17,etc.),and another 1 was without a clear chromosomal localization.The transmembrane structure prediction indicated that the NtPILS protein contained 7 to 10 transmembrane structures.The promoter element analysis revealed that the NtPILS gene was rich in non-biological stress response,light response,and hormone response elements.The 14 NtPILS genes showed tissue-specific expression in different or-gans of Nicotiana tabacum(roots,stems,leaves,flowers,etc.).Most of the NtPILS genes respon-ded to low temperature,drought and salt stress.

关键词

普通烟草/生长素/PILS基因家族/非生物胁迫

Key words

Nicotiana tobacum/Auxin/PILS gene family/abiotic stress

分类

农业科技

引用本文复制引用

刘翀,梁书苑,王飞雁,刘洋,鲍亚宁..烟草PILS基因家族鉴定及表达模式分析[J].种子,2026,45(1):1-11,11.

基金项目

贵州省科技计划项目"贵州省烟草提质增效全省重点实验室"(黔科合平台ZSYS[2025]028) (黔科合平台ZSYS[2025]028)

种子

1001-4705

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