种子2026，Vol.45Issue(1)：58-66,9.DOI:10.16590/j.cnki.1001-4705.2026.01.058
联合转录组学与代谢组学探究嫁接对茶树脂质代谢的影响
Joint Transcriptomics and Metabolomics Revealing the Effect of Grafting on Lipid Metabolism in Plants of Camellia sinensis
摘要
Abstract
In order to explore the effect of grafting on the lipid metabolism in Camellia sinensis plants,the Yuntao variety of Camellia sinensis with 1 bud and 2 leaves after grafting and the Yuntao variety of Camellia sinensis that had not undergone grafting were used as test materials.Using transcriptom-ics and metabolomics techniques in combination,the mechanism of lipid substance changes of Camel-lia sinensis after grafting was analyzed.The results showed that 2 281 differentially expressed genes and 105 differentially expressed metabolites were screened out for both the grafted Yuntao and the un-grafted Yuntao plants of Camellia sinensis.After grafting,980 genes in the plants of Camellia sinen-sis showed increased expression levels,while 1 301 genes showed decreased expression levels.The content of 61 metabolites increased,and 44 metabolites decreased.Among them,the content of 15 lipid metabolites increased,and 1 decreased.Through the KEGG pathway enrichment analysis of transcriptomics and metabolomics,732 differentially expressed genes and 24 differentially expressed metabolites were annotated to 30 pathways.These pathways mainly include metabolic pathways,bio-synthesis of secondary metabolites,and biosynthesis of phenylpropanoids,etc.In the glycerophos-pholipid metabolism pathway,lipid substance glycerophosphate choline GPC and 8 differentially ex-pressed genes such as LOC114266827 had a certain regulatory relationship;in the ether lipid metabo-lism pathway,lipid substance glycerophosphate choline GPC and 4 differentially expressed genes such as LOC114259844 had a certain regulatory relationship.关键词
茶树/嫁接/脂质代谢物/代谢组学/转录组学Key words
Camellia sinensis/graft/lipid metabolites/metabolomics/transcriptomics分类
农业科技引用本文复制引用
周泳臣,高峻,曾庆群,胡志宏,常润润,李媛媛,赵红艳,严慧婷,王艳燕,刘志薇..联合转录组学与代谢组学探究嫁接对茶树脂质代谢的影响[J].种子,2026,45(1):58-66,9.基金项目
"十四五"国家重点研发计划重点专项(2022YFD1601803) （2022YFD1601803）
云南省农业农村厅科技专项(2018KJTX007) （2018KJTX007）
