种子2026，Vol.45Issue(1)：66-78,87,14.DOI:10.16590/j.cnki.1001-4705.2026.01.066
小麦盐胁迫抗性相关bHLH转录因子生物信息学分析与功能预测
Bioinformatics Analysis and Functional Prediction of bHLH Transcription Factors Related to Salt Stress Resistance of Wheat
摘要
Abstract
The bHLH transcription factors play important roles in the responses of crops to environ-mental stress and during their growth and development.To further study the functions of bHLH transcription factors in the salt stress response of wheat(Triticum aestivum L.),transcriptome se-quencing was conducted on the salt-tolerant material Cangmai6005 and the salt-sensitive material Kenong9204.The salt-related bHLH transcription factors were screened and subjected to bioinformat-ics analysis.The tested materials were treated with salt stress,and RNA was extracted from the root meristematic tissues.The cDNA was reverse-transcribed,and fluorescence quantitative PCR was per-formed to verify 11 key candidate genes.The results showed that a total of 33 salt-related bHLH tran-scription factors were screened out,and the encoded proteins were mainly located in the nucleus.The secondary structure was mainly composed of random coils and a-helices.The proteins were all hydro-philic.The phosphorylation sites were mainly serines.The promoter regions contained various ele-ments related to hormone response and stress induction.The analysis of the temporal and spatial ex-pression patterns of the candidate genes indicated that they were expressed in certain tissues,with the root being the main site.After salt stress treatment,the expression levels of 2 out of 11 key candidate genes significantly increased,and 2 genes significantly decreased,which was consistent with the re-sults of the transcriptome sequencing.These genes could mediate the salt tolerance of wheat.关键词
小麦/盐胁迫/bHLH/转录因子/生物信息学分析Key words
Triticum aestivum/salt stress/bHLH/transcription factors/bioinformatics analysis分类
农业科技引用本文复制引用
罗政辉,王伟伟,张玉杰,王志,邹景伟,赵振杰,魏晨曦,钮力亚,于亮,王奉芝..小麦盐胁迫抗性相关bHLH转录因子生物信息学分析与功能预测[J].种子,2026,45(1):66-78,87,14.基金项目
国家自然科学基金项目(U24A20391) （U24A20391）
河北省自然科学基金重大专项(C2023205050) （C2023205050）
国家小麦产业技术体系沧州综合试验站(CARS03-2) （CARS03-2）
